The effect of mosquito midgut trypsins in dengue serotype 2 flavivirus (DENV-2) infectivity to Aedes aegypti was studied. Addition of soybean trypsin inhibitor (STI) in a DENV-2 infectious blood meal resulted in a 91-97% decrease in midgut DENV-2 RNA copies (qRT-PCR analysis). STI treatment also resulted in slower DENV-2 replication in the midgut, less DENV-2 E protein expression, and decreased...

The most vulnerable stages of Plasmodium development occur in the lumen of the mosquito midgut, a compartment shared with symbiotic bacteria. Here, we describe a strategy that uses symbiotic bacteria to deliver antimalaria effector molecules to the midgut lumen, thus rendering host mosquitoes refractory to malaria infection. The Escherichia coli hemolysin A secretion system was used to promote ...

Mosquito midgut microbiota is a key component of vector competence, as gut bacteria can disturb pathogen development. In this study, we addressed the microbiota composition of Aedes aegypti during its lifespan, under field conditions. We also investigated the possible effects of environment, dietary regime and ageing on the gut community composition. We employed culture independent and dependen...

BACKGROUND Aedes aegypti is the primary mosquito vector for dengue virus (DENV) worldwide. Infectivity of dengue virus varies among natural populations of this mosquito. How A. aegypti responds to DENV infection relative to which genes and associated pathways contribute to its differential susceptibility as a vector is not well defined. METHODS/PRINCIPAL FINDINGS Here, we used custom cDNA mic...

Secreted ferritin in the mosquito, Aedes aegypti, has several subunits that are the products of at least two genes, one encoding a homologue of the vertebrate heavy chain (HCH) and the other the light chain homologue (LCH). Here we report the developmental and organ specific pattern of expression of the ferritin HCH messages and of both subunit types in control sugar-fed mosquitoes, in those ex...

The value of protozoa in investigations of nucleocytoplasmic relationships has long been appreciated, as indicated by reviews (1, 3, 7) of the extensive literature on this subject. Though the malarial parasite apparently has not previously been employed in such studies, we have found it to be a potentially useful organism in this regard. The elaborate differentiation that the parasite undergoes...

This review briefly focuses on the role of mosquito salivary glands; and on the biological processes and mechanisms relevant to transmission of malarial parasite (Plasmodium), the causative agent for malaria. A key requirement for transmission of the parasite is an infected blood meal which initiates parasite transmission cycle. The blood feeding is an organized biological mechanism which invol...

Cry4Aa toxin produced by Bacillus thuringiensis subsp. israelensis exhibits specific toxicity to larvae of medically important mosquito genera. In the present study, we analyzed characteristics channel pores formed recombinant in a solvent-free planar lipid bilayer. Stable currents were observed electrophysiologic measurements, and single-channel conductance was 187 ± 10 pS symmetrical buffer c...

Mosquitoes are considered as the most nuisance causing vectors they responsible for a widespread of diseases. Malaria-borne mosquitoes killing two to three million people and infect an estimated hundred or more annually. A host other mosquito-borne diseases including filariasis, yellow fever, dengue, encephalitis kill debilitate hundreds millions more. The present study investigates bio efficac...