Objective(s): Here, we examined the function of our produced monoclonal antibody (mAb10C3) to recognize one of the most important members of the HEAT shock factor family, Hsf5, in embryonic development and in spermatogenic cells of adult mouse testis.Materials and Methods: The targeting effects of mAb10C3 were investigated by immunohisto...

background: the basis of spermatogenesis is the spermatogonial stem cells (sscs). the concentration of sscs is very small. however, a system that supports the proliferation and maintenance of sscs in vitro could be used to preserve and expand sscs numbers as well as increase success in transplantation. it is a new avenue to restore spermatogenesis in azoospermia subjects. objective: proliferati...

The proper regulation of spermatogenesis is crucial to ensure the continued production of sperm and fertility. Here, we investigated the function of the H3K4me2 demethylase KDM1A/LSD1 during spermatogenesis in developing and adult mice. Conditional deletion of Kdm1a in the testis just prior to birth leads to fewer spermatogonia and germ cell loss before 3 weeks of age. These results demonstrate...

Development of the mammalian testis and spermatogenesis involve complex processes of cell migration, proliferation, differentiation, and cell-cell interactions. Although our knowledge of these processes has increased in the last few decades, many aspects still remain unclear. The lack of suitable systems that allow to recapitulate and manipulate both testis development and spermatogenesis ex si...

We investigated the role of oxidative stress-responsive kinase-1 (OSR1) and STE20 (sterile 20)/SPS1-related proline/alanine-rich kinase (SPAK), upstream regulators of the Na+-K+-2Cl- cotransporter (NKCC1)-essential for spermatogenesis-in mouse models of male fertility. Global OSR1+/- gene mutations, but not global SPAK-/- or Sertoli cell (SC)-specific OSR1 gene knockout (SC-OSR1-/-), cause subf...

Spermatogenesis is a complex differentiation process that involves genetic and epigenetic regulation, sophisticated hormonal control, and extensive structural changes in male germ cells. RNA nuclear and cytoplasmic bodies appear to be critical for the progress of spermatogenesis. The chromatoid body (CB) is a cytoplasmic organelle playing an important role in RNA post-transcriptional and transl...