Grass pea (Lathyrus sativus L.) is highly nutritious and protein rich legume crop; however, presence of ODAP (β-NOxalyl-L-α, β-diaminopropionic acid) has restricted its use for human and animal consumption. There is need for accelerated breeding of low or zero ODAP cultivars to ensure safer and wider use of grass pea through conventional and biotechnological approaches. The study investigated s...

Carob tree ( Ceratonia siliqua L.) is an agro-sylvo-pastoral species that has been classified among the most efficient trees. The first publications on " in-vitro multiplication of are very recent and show conflicting results. Mastery this technique seems necessary to provide increased demand international market for gum extracted from endosperm seed, a polyoside called galactomannan. This work...

Ficus benjaminais a very popular and resistant indoor plant in temperate areas. Cutting is the easiest propagation method of this plant and it requires special treatments such as auxin and appropriate rooting medium. In order to test the suitable auxin (IBA) concentration and rooting media, a factorial experiment carried out based on randomized complete block design with three replications. Exp...

Aloe vera is an important medicinal perennial herb belonging to the family liliaceae. A method for mass propagation of Aloe vera by using different explants and different media with different PGR contain has been developed. Two type of explants (with and without sheath Type A and B respectively) were cultured on MS, B5 and SH media supplemented with different combination of different NAA with B...

The effect of plant growth regulators on shoot proliferation from shoot tip explants of Ajuga multiflora was studied. The highest number of shoots (17.1) was observed when shoot tip explants were cultured on Murashige and Skoog (MS) medium fortified with 8.0 µM 6-Benzyladenine (BA) and 2.7 µM α-naphthaleneacetic acid (NAA). The mean number of shoots per explant was increased 1.6-fold in liquid ...

In breeding programs, a large number of F₂ individuals are required to perform the selection process properly, but often few such plants are available. In order to obtain more F₂ seeds, it is necessary to multiply the F₁ plants. We developed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of seeds using 6-benzylaminopurine. To optimize shoot regeneration...

Pterocarpus santalinus belongs to fabaceae family and is one of the endemic and endangered medicinal plant species of seshachalam hills, Tirumala, Andhra Pradesh state, India. In the present study we have successfully demonstrated the in vitro regenartion of this plant through shoot bud culture. The seeds germinated sequentially on plastic trays and transferred polythene bags for 21 days served...

A complete protocol is presented for in vitro regeneration of pomegranate (Punica granatum L.), a tropical fruit tree, using cotyledonary nodes derived from axenic seedlings. Shoot development was induced from cotyledonary nodes on Murashige and Skoog (1962) (MS) medium supplemented with 2.3±23.0 mM benzyladenine (BA) or kinetin (Kn). Both type and concentration of cytokinin signi®cantly in ̄uen...

This study describes a Platycerium bifurcatum (Cav.) C. Chr. propagation method through initiation of green globular bodies (GGBs) from juvenile leaf explants of in vitro grown sporophytes. The GGB initiation was first obtained by incubating the explants on MS medium supplemented with α-naphthalene acetic acid (NAA) and two other cytokinins, 2-isopentenyl adenine (2ip) or 6-benzylaminopruine (B...