Infections caused by Chlamydia spp are an important cause of human disease, and the accuracy and reproducibility of antimicrobial susceptibility tests for these bacteria could have considerable clinical implications. We have developed a reverse transcriptase PCR (RT-PCR) based method to determine the antibiotic susceptibility of Chlamydia spp., and compared this with conventional tests using im...

To gain information on retrotransposons in the genome of Paragonimus westermani, PCR was carried out with degenerate primers, specific to protease and reverse transcriptase (rt) genes of long-terminal-repeat (LTR) retrotransposons. The PCR products were cloned and sequenced, after which 12 different retrotransposon-related sequences were isolated from the trematode genome. These showed various ...

Successful disease management requires a rapid and sensitive diagnosis method that can recognize early infection even before the manifestation of its clinical signs. The only available field diagnostic tests for foot-and-mouth disease (FMD) are lateral flow devices, commonly known as chromatographic strips. Low sensitivity and inability to detect FMD virus (FMDV) at the serotype level are limit...

Substrate properties of various morpholinonucleoside triphosphates in the reaction of DNA elongation catalyzed by DNA polymerase beta, reverse transcriptase of human immunodeficiency virus (HIV-1 RT), and reverse transcriptase of Moloney murine leukemia virus (M-MuLV RT) were compared. Morpholinonucleoside triphosphates were utilized by DNA polymerase beta and HIV-1 reverse transcriptase as sub...

Objectives To examine the interactions between SARS-CoV-2 positive players and other during rugby league matches determine within-match transmission risk. Methods Four Super League in which were subsequently found to have participated analysed. Players identified as increased-risk contacts, player proximities analysed by video footage global positioning system (GPS) data. The primary outcome wa...

Diagnosis of respiratory virus infections currently involves detection by isolation or antigen detection, which usually identifies only a single suspected agent. To permit identification of more than one respiratory virus in clinical specimens, a rapid detection method involving a single-step, multiplex reverse transcription-PCR (RT-PCR) assay was developed. The assay included five primer sets ...

OBJECTIVE To examine whether human endogenous retrovirus K10 is associated with autoimmune rheumatic disease. DESIGN A novel multiplex reverse transcription polymerase chain reaction (RT-PCR) system was developed to investigate HERV-K10 mRNA expression in patients with rheumatoid arthritis. METHODS 40 patients with rheumatoid arthritis, 17 with osteoarthritis, and 27 healthy individuals wer...

Aim: To evaluate the role of high-resolution Computer tomography imaging in management COVID-19. Study design: Prospective study Place and duration study: Jinnah Postgraduate Medical Centre Karachi from 1st December 2021 to 31st May 2022. Methodology: One hundred patients suspected be suffering COVID-19 were enrolled. All underwent Reverse transcriptase-based polymerase chain reaction tests (RT...