MMR vaccine failure had been reported globally and here we report that it occurs now in India. Samples were collected from clinically suspected mumps cases were subjected for anti mumps antibodies, virus isolation, RT-PCR, sequencing and phylogenetic tree analysis. 56 samples collected from men and women belonging to various age groups. 30 had been vaccinated and the status of 26 patients was u...

Guinea pigs experimentally infected with mumps virus develop a delayed, hypersensitive skin reaction following the intradermal injection of heat-inactivated mumps virus. This in vivo hypersensitivity is accompanied by a state of cellular hypersensitivity which can be demonstrated in vitro by the addition of mumps viral antigen to cultures of splenic macrophages, following which they become less...

Here, we report a case of a 51-year-old man with acute pericardial tamponade requiring emergency pericardiocentesis after he suffered from sore throat, headache, malaise, and sweats for two weeks. Serological analyses revealed increased mumps IgM and IgG indicating an acute mumps infection whereas other bacterial and viral infections were excluded. In addition, MRI revealed atypical swelling of...

Two enzyme immunoassays (ELISA) for mumps antibody detection using the Enzygnost (Germany) and Parotit-screen (Russia) were comparatively assayed using sera of randomly assigned 70 healthy young adult volunteers. The neutralization test (NT) was performed for all sera using mumps viruses (MVs) of the relevant strains Enders and Leningrad-3. The proportion of positive results was significantly h...

OBJECTIVE To evaluate the adequacy of measles, mumps and rubella (MMR) vaccination among children and to assess risk of outbreak in Jeddah, Kingdom of Saudi Arabia (KSA). METHODS We recruited a total of 527 children from schools located at different urban regions, and preschool children from those attending the well baby clinics in Jeddah, KSA. We tested for MMR viruses antibodies using ELISA...

Following a national mumps outbreak that began in November 2004 and continued into 2005, the number of mumps notifications in Ireland waned in the latter half of 2006 and during 2007 (Figure 1). However, mumps notifications have started to increase again in 2008 (Figure 1). The number of mumps notifications annually between 1988 and 2008 and the number of confirmed mumps notifications by year f...

Mumps virus-neutralizing antibodies are believed to be the most predictable surrogate marker of protective immunity. However, assays used to detect neutralizing antibodies, such as the plaque reduction neutralization (PRN) assay, are labor- and time-intensive and consequently are often supplanted by the more rapid and inexpensive enzyme immunoassay (EIA) technique. For virus infections for whic...

A sensitive nested reverse transcription-PCR assay, targeting a short fragment of the gene encoding the small hydrophobic protein (SH gene), was developed to allow rapid characterization of mumps virus in clinical samples. The sensitivity and specificity of the assay were established using representative genotypes A, B, C, D, E, and F. Mumps virus RNA was characterized directly from cerebrospin...

In 2006, nearly 6,000 mumps cases were reported in the United States, 795 of which occurred in Illinois. In Chicago, 1 healthcare institution experienced ongoing transmission for 4 weeks. This study examines the outbreak epidemiology and quantifies the financial affect on this organization. This retrospective cohort study was conducted through case and exposure identification, interviews, medic...