Nontuberculous mycobacteria are often underdiagnosed due to lack of proper diagnostic facilities. To overcome this, we created a rapid PCR method for the species-specific diagnosis of Mycobacterium tuberculosis and its differentiation from other mycobacteria. A set of PCR primers targeting the gene encoding for early-secreted antigen-6 (ESAT-6) of the M. tuberculosis complex was designed and st...

Epichloe typhina is an endophytic fungus, while Cladosporium phlei is a pathogenic fungus of the timothy plant (Phleum pretense L.). We found two activities in the culture filtrate of E. typhina: one stimulated the pathogenic fungus, C. phlei, to produce phleichrome and the other inhibited its growth. The active ingredients that stimulated the production of phleichrome and inhibited the growth ...

The Non-Tuberculous Mycobacteria (NTM),whose habitat the environment has acquired importance in the last years because of the immunosupressed patients, infected HIV ,and also in develop countries that have managed to eradicated the bovine tuberculosis. Where it has been verified that certain NTM interfere in the diagnosis of tuberculosis when it is applied to the delayed hypersensitivity test w...

The NN-dicyclohexylcarbodiimide (DCCD). sensitive latent adenosinetriphosphatase (ATPase)(EC 3.6.1.3; ATP phosphohydrolase) from Mycobacterium phlei has been purified to homogeneity and used to restore oxidative phosphorylation to detergent-extracted membranes. The phosphorylation was inhibited by DCCD and by tetraphenylboron and valinomycin. The enzyme was solubilized from the membrane vesicle...