BACKGROUND Gold nanoparticles have been widely employed for biosensing purposes with remarkable efficacy for DNA detection. Amongst the proposed systems, colorimetric strategies based on the remarkable optical properties have provided for simple yet effective sequence discrimination with potential for molecular diagnostics at point of need. These systems may also been used for parallel detectio...

1 The State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. of China. 2 College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, P. R. of China. 3 State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. of ...

In the context of a busy reference laboratory, radiometric selective inhibition tests were evaluated for rapid differentiation of Mycobacterium tuberculosis and Mycobacterium bovis and of the M. tuberculosis complex from other mycobacteria. p-Nitro-alpha-acetylamino-beta-hydroxypropiophenone at 5 micrograms and hydroxylamine hydrochloride at 62.5 and 125 micrograms per ml of 7H12 medium were us...

The Seeplex TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the Mycobacterium tuberculosis complex (MTBC) targets IS6110 and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specimens, the sensitivity and specificity of this assay is equivalent to a labor-intensive traditional e...

We selected 180 clinical isolates of the Mycobacterium tuberculosis complex (MTBC) from patients in China and performed comparative sequence analysis of the mpt64 gene after amplification. From the results, we found that polymorphisms of the mpt64 gene in the MTBC may be the reason for changes in the antigen produced, which may in turn cause alterations of related functions, thereby allowing im...

*Unité de Génétique Moléculaire Bactérienne, ‡Laboratoire de Génomique des Microorganismes Pathogènes, and §Centre National de Référence des Mycobactéries, Institut Pasteur, 25-28 Rue du Docteur Roux, 75724 Paris Cedex 15, France; †Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom; ¶Mycobacteria Reference Department, Diagnostic Laboratory for Inf...

Evolution of the direct repeat region in Mycobacterium tuberculosis has created unique spoligotype signatures specifically associated with IS6110-defined strain families. Spoligotyping signatures may enable the analysis of the strain population structure in different settings and will enable the rapid identification of strain families that acquire drug resistance or escape protective immunity i...

This chapter highlights current state-of-the-art methods for the detection and identification of Mycobacterium tuberculosis (Mtb) complex in the clinical diagnostic laboratory. Methods discussed include stain and culture which traditionally would have been followed by phenotypic-based identification methods. At this point in time however, molecular methods are considered the gold standard for b...

background and objectives: diagnosis of avian tuberculosis by conventional culture method is still considered as the “gold standard” technique. the main objective of this study was to compare growth of mycobacterium avium subsp. avium on four specific mycobacterial cultures such as glycerinated lowenstein-jensen medium, pyruvate-enriched lowenstein- jensen medium, mycobactin j-supplemented herr...