Po protein, the dominant protein in peripheral nervous system myelin, was studied immunocytochemically in both developing and mature Schwann cells. Trigeminal and sciatic nerves from newborn, 7-d, and adult rats were processed for transmission electron microscopy . Alternating 1-lam-thick Epon sections were stained with paraphenylenediamine (PD) or with Po antiserum according to the peroxidase-...

The myelin sheath insulates axons and allows for rapid salutatory conduction in the nervous system of all vertebrates. The formation of peripheral myelin requires expression of the transcription factor Egr2, which is responsible for inducing such essential myelin-associated genes as Mpz, Mbp, Pmp22, and Mag. Using microarray analysis to compare gene expression patterns in peripheral nerve durin...

We analyzed the role of Fyn tyrosine kinase in CNS myelination by using fyn(-/-) null mutant mice, which express no Fyn protein. We found a severe myelin deficit in forebrain at all ages from 14 d to 1 year. The deficit was maximal at 1 month of age and was similar regardless of mouse strain background or whether it was determined by bulk isolation of myelin or by quantitation of myelin basic p...

by the decrease in incorporation of labelled methyl groups from enzymically synthesized S-adenosylmethionine into basic protein by spinal-cord protein methylase activity (Table 1). Electrophoresis of the labelled protein precipitate on acetic acidhrea-polyacrylamide gels (Deibler et al., 1972), followed by autoradiography of the gels, indicated that only the myelin basic protein was detectably ...

Experimental allergic encephalomyelitis is characterized by invasion of lymphocytes and macrophages into the central nervous system resulting in inflammation, edema, and demyelination. Sera from Lewis rats from 7-95 days after immunization with purified guinea pig CNS myelin were examined with respect to their ability to opsonize myelin. This was correlated with the appearance of antibody compo...

Myelin is essential in vertebrates for the rapid propagation of action potentials, but the molecular mechanisms driving its formation remain largely unknown. Here we show that the initial stage of process extension and axon ensheathment by oligodendrocytes requires dynamic actin filament assembly by the Arp2/3 complex. Unexpectedly, subsequent myelin wrapping coincides with the upregulation of ...

Our previous study reported the translocator protein to play a critical role in neuropathic pain and the possible mechanisms in the spinal cord. However, its mechanism in the peripheral nervous system is poorly understood. This study was undertaken to explore the distribution of translocator protein in the dorsal root ganglion and the possible mechanisms in peripheral nervous system in a rat mo...

The corpus callosum (CC) is the largest interhemispheric commissure of mammalian brain, and it includes axons, cortical neurons, glial cells. It mainly composed myelin, a lipidic sheath which produced by cell membranes; myelin wrapped up around axons plays fundamental role in fast conduction neuronal electrical signals. human CC divided into various anatomical regions, with different axonal com...

The distribution of newly synthesized proteolipid protein (PLP, 23 kdaltons) and myelin basic proteins (MBPs, 14-21.5 kdaltons) was determined in microsomal and myelin fractions prepared from the brainstems oi 10-30 d-old rats sacrificed at different times after an intracranial injection of 3~S-methionine. Labeled MBPs were found in the myelin fraction 2 min after the injection, whereas PLP app...

background:   valproic acid (vpa) is used to be an effective anti-epileptic drug and mood stabilizer. it has recently been demonstrated that vpa could promote neurite outgrowth, activate the extracellular signal regulated kinase pathway, and increases bcl-2 and growth cone-associated protein 43 levels in spinal cord. in the present research we demonstrate the effect of vpa on peripheral nerve r...