Two classes of RNA ligands that bound to separate, high affinity nucleic acid binding sites on Qj8 replicase were previously identified. RNA ligands to the two sites, referred to as site I and site II, were used to investigate the molecular mechanism ofRNA replication employed by the four-subunit replicase. Replication inhibition by site Iand site 1I-specific ligands defined two subsets of repl...

I �as sur�rise�,� t� �ut it li�htly,� t� rea� in a recent letter (htt�://�nline.�sj.c�m/article/SB10001424052970204301404 577171531838421366.html?m��=WSJ_O�ini�n_LEADT��) t� the Wall Street Journal that,� “There is n� c�m�ellin� scientific ar�ument f�r �rastic acti�n t� ‘�ecarb�nize’ the ��rl�’s ec�n�my.” The letter �as si�ne� by 16 scientists fr�m several �resti�i�us instituti�ns,� inclu�in� P...

The peptide mating pheromone a-factor and the hydrolytic enzyme invertase (f3D-fructofuranoside fructohydrolase, EC 3.2.1.26) are processed from larger precursor proteins during their secretion from yeast cells (Saccharomyces cerevisiae). An in-frame fusion of the structural genes for these two proteins was constructed by connecting the 5'-flanking region and preproleader portion of the coding ...

Eighteen overlapping cosmid clones spanning 240 kilobases and encoding the gene for factor B and two genes related to the fourth component of complement (C4) were isolated from a murine H-2d genomic library. Cosmid clones were identified by hybridization to human cDNA probes for factor B and C4 and were linked by chromosomal walking procedures. The cluster of clones contains two regions with se...

The Third Viennese School of Psychotherapy, as Viktor E. Frankl’s Logotherapy is commonly hailed, veers around the proposition that the primary motivational force of human existence is neither ‘will to pleasure’ as propounded by Psychoanalysis nor ‘will to power’ of Adlerian concept but a sheer ‘will to meaning’. Logotherapy encapsulates the Greek word ‘logos’ in the sense of meaning, thus maki...

This report describes a method for growing both bloodstreamand procyclic-form Trypanosoma brucei as colonies on agarose plates. Procyclic colonies, which took 2 weeks to develop, grew with approximately 17% plating efficiency on SDM-79/0.65% agarose supplemented with 20% (vol/vol) conditioned medium. Bloodstream forms were adapted to in vitro growth in liquid HM-9 medium and then spread on HMI-...

s David Alonso-Gutiérrez On a different proof of Junge’s estimate for the isotropy constant of polytopes Tuesday 4:30–4:55 p.m. Abstract: It was proved by M. Junge that the isotropy constant of any symmetric polytope with 2N vertices is bounded by C logN . We give a different proof of this result, which shows that the same estimate is true when the polytope is nonsymmetric with N vertices. It w...

Variations in unit-cell volumes of mantle minerals as functions of P and T are important parameters in the description of the interior of the Earth and the behavior of materials. Recently, Thompson and Downs (2004) presented a model for the crystal structures of pyroxenes parameterized in terms of the O3-O3-O3 angle, θ, and the oxygen radius, r. This model has proven useful in the analysis of c...

Stimulated human monocytes/macrophages are a source of mediators such as tumor necrosis factor a (TNF-a), interleukin 1 (IL-1), and prostaglandin E2 (PGE2), which can modulate inflammatory and immune reactions. Therefore, the ability to control the production of such mediators by monocytes/macrophages may have therapeutic benefits, and it has been proposed that glucocorticoids may act in this w...

Pharmacokinetic characteristics of INH are presented. Adverse effects and toxicity caused by INH has been reviewed under the headings of neuropathy and seizures, hepatotoxicity, rhabdomyolysis, agranulocytosis, acidosis and other adverse drug reactions. Cases of poisoning by INH have been mentioned independently. Few case reports indicating symptoms of intoxication and the mechanism of toxicity...