Species diversity is largely underestimated by current taxonomy, precluding a precise understanding of evolutionary processes. Genetic data have increased our understanding of that cryptic diversity, and multilocus studies are now desirable. In this study, we used mitochondrial and nuclear DNA sequences to evaluate the taxonomic status of the western Mexico's populations of Phaethornis longiros...

The diagnosis of primary mitochondrial myopathy is often delayed by years due to non-specific clinical symptoms as well variable testing disorders. aim this review summarize and discuss the collective findings novel insights regarding diagnosing, testing, presentation (PMM). PMM results from a disruption oxidative phosphorylation (OXPHOS) chain in mitochondria mutations DNA (mtDNA) or nuclear (...

We aimed to extract DNA and amplify PCR fragments at the mitochondrial Nad7.1 locus 11 nuclear microsatellite loci in nine circa 11,000-year-old individuals of Scots pine found bottom Baltic sea test genetic associations with present-day gene pool Lithuania. followed a strict anticontamination protocol lab and, simultaneously aDNA specimens, tested DNA-free controls. The was extracted by an ATM...

BACKGROUND AND AIMS Understanding the introduction history of invasive plant species is important for their management and identifying effective host-specific biological control agents. However, uncertain taxonomy, intra- and interspecific hybridization, and cryptic speciation may obscure introduction histories, making it difficult to identify native regions to explore for host-specific agents....

BACKGROUND Anisakiasis is a fish-borne zoonosis caused by Anisakis spp. larvae. One challenging issue in the diagnosis of anisakiasis is the molecular detection of the etiological agent even at very low quantity, such as in gastric or intestinal biopsy and granulomas. Aims of this study were: 1) to identify three new cases of invasive anisakiasis, by a species-specific Real-time PCR probe assay...

The purpose of the work is to assess state Anura populations living in an anthropo-genic territory according a set indicators body’s immune homeostasis. objects study were Pelophylax ridibundus (Pallas, 1771) and P. lessonae (Camerano, 1882), natural reservoirs Nizhny Novgorod. Priority chemical pollutants water bodies determined by spectrophotometry. Species identified using multiplex PCR test...

The accurate quantification of DNA in forensic samples is utmost importance. These are often present limited amounts; therefore, it indicated to use the appropriate analysis route with optimum amount (when possible). Also, can inform about degradation stage and therefore support decision on which downstream genotyping method use. Consequently, aids getting best possible results from a sample, c...

Background Thyroid disease is a common complication of systemic lupus erythematosus manifesting in 30% cases due to disturbance autoimmune mechanisms. Objectives evaluating the effect thyroid lesion on course based clinical and laboratory parameters, level antibodies hormones. Methods Among 65 examined patients with SLE (63 women 2 men aged 19-65) we revealed disorder third patients. Patients w...