Negative stain electron microscopy (EM) allows relatively simple and quick observation of macromolecules and macromolecular complexes through the use of contrast enhancing stain reagent. Although limited in resolution to a maximum of ~18 - 20 Å, negative stain EM is useful for a variety of biological problems and also provides a rapid means of assessing samples for cryo-electron microscopy (cry...

When people understand noun-noun compounds such as kitchen mirror they generate a relationship between the two constituent nouns, combining them together in a new concept. How do people determine or construct the correct relational link between the constituents? In our first experiment, we present evidence against one approach to noun-noun compounds, namely that of specifying the meaning of a c...

An evaluation of the standardised Romanowsky stain of Marshall et al. has been made in a routine haematology laboratory. It was noted that this stain had several advantages over the May-Grünwald Giemsa stain used in most British laboratories. These advantages include ease and speed of preparation, a shorter staining time, and reproducibility of results. These results are described in detail. Th...

UTILITY: (1) Assess degree of fibrosis. H&E stain is not reliable for demonstration of fibrosis especially in early stages. Pericellular fibrosis around the central vein in steatohepatitis can be difficult to identify on the H&E stain. (2) Distinguish fibrosis from necrosis. In acute hepatitis, areas of confluent necrosis can be difficult to distinguish from fibrosis and can lead to an erroneou...

We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain ...

Background and Aim: Management of iron-induced tooth pigmentation is challenging. This study aimed to compare the efficacy of sodium bicarbonate powder and pumice flour for removal of iron-induced pigmentation from primary ante-rior teeth. Materials and Methods: In this in vitro, experimental study, 60 sound primary anterior teeth were collected. After cutting the roots and sealing the pulp ch...

The recent paper by Rotimi and colleagues 1 does not mention the acridine orange stain 2 when comparing staining methods for the identification of Helicobacter pylori. The acri-dine orange stain uses ultraviolet fluores-cence in the identification of bacteria. The typical curved morphology of H pylori can easily be diVerentiated from other bacteria. I have used this quick, cheap, and reliable s...

The effects of fluorescein and Richardson's stain on corneal epithelial wound healing were compared in eyes of rabbits whose corneas had the epithelium removed by scraping or by n-heptanol. One eye of each rabbit was stained with fluorescein and the other eye was stained with Richardson's stain at intervals throughout the healing process, and the wounds were photographed for planimetry and dete...