Lidocaine has been shown to inhibit the invasion and metastasis of breast cancer, but mechanism still remains unclear. This study explored relationship between lidocaine circulating seeding cancer cells from perspective nerve fiber formation. The cell lines MDA-MB-231 4T1 were subcutaneously inoculated in mice simulate tumor self-seeding by cells. was used treat these growth observed. Silver st...

The identities and quantities of calcium-binding proteins were determined in axoplasm isolated from the squid giant axon. 45Ca-binding assays on nitrocellulose filters containing axoplasm proteins separated by SDS-polyacrylamide electrophoresis revealed 4 major calcium-binding bands. These included the high-molecular-weight (Mr greater than 330 and 220 X 10(3] neurofilament proteins, an unident...

To analyze the possible influence of nerve growth factor (NGF) on neurofilament synthesis in primary sensory neurons, adjacent cryostat sections of lumbar dorsal root ganglia (DRG) from adult rats were processed for either NGF-receptor radioautography or in situ hybridization with a neurofilament cDNA probe. Labeling by both procedures was quantified with computer assistance for approximately 3...

The control of radial growth of axons is of functional importance because caliber is a principal determinant of conduction velocity in myelinated nerve fibers. Neurofilaments, the major cytoskeletal protein in myelinated nerves, appear to be intrinsic determinants of caliber. Evidence supporting this derives first from the linear relationship between neurofilament content and axonal diameter. F...

The structural role of neurofilaments in the normal axon and the consequences of altered axonal transport of neurofilaments have been extensively studied in large axons. These studies suggest that neurofilament numbers and interneurofilament spacing are major determinants of axonal crosssectional area. In contrast, in small axons and dendrites, microtubules and membranous organelles appear to b...

Dorsal root ganglia (DRG) are recognized as one of the organs which are damaged in peripheral sensory diabetic neuropathy. In an experimental animal model, the alteration of the mRNA expression level of neurotrophins, their receptors and neuronal cytoskeletal protein have been reported. In this study, we examined whether these changes are improved by treatment with the aldose reductase inhibito...

Using anti-chicken brain neurofilament antisera, Alzheimer's neurofibrillary tangles from two patients with senile dementia were stained by immunofluorescence and by the peroxidase-antiperoxidase procedure in cryostat sections of hippocampus and frontal cortex. In sections of cerebellum obtained from the same patients, the distribution of immunostaining was the same as that observed in experime...

The effect of a single 750-mg/kg oral dose of tri-o-cresyl phosphate (TOCP) on the endogenous phosphorylation of brain microtubule preparations and spinal cord neurofilaments was assessed in hens after the development of delayed neurotoxicity. Protein phosphorylation with [gamma-32P]ATP was analyzed by one-dimensional and two-dimensional gel electrophoresis, autoradiography, and microdensitomet...

AIMS We have previously identified neurofilament-protein-containing neurites in human epiretinal membranes (ERMs). The aim of this study was to further characterise these neurites by examining the expression of additional specific proteins in human ERMs and to correlate this expression with various retinal disease conditions. METHODS Epiretinal membranes originating from 43 patients with prol...

Alterations in neurofilaments are a common occurrence in neurons of the human nervous system during aging and diseases associated with aging. Such pathologic changes may be attributed to species-specific properties of human neurofilaments as well as cell-type-specific regulation of this element of the cytoskeleton. The development of transgenic animals containing human neurofilament subunits of...