The size of tissue amino acid pools in plants may indicate nitrogen status and provide a signal that can regulate nitrate uptake and assimilation. The effects of treating barley roots with glutamine have been examined, first to identify the transport system for the uptake of the amino acid and then to measure root NR activity and cellular pools of nitrate. Treating N replete roots with glutamin...

Nitrate is an important component of the nitrogen cycle and is therefore present in all plants. However, excessive nitrogen fertilization results in a high nitrate content in vegetables, which is unhealthy for humans. Understanding the spatial distribution of nitrate in leaves is beneficial for improving nitrogen assimilation efficiency and reducing its content in vegetables. In this study, nea...

As natural plant growth stimulators, amino acids are widely used to improve the yield and quality of crops. Change in enzymes activities of N assimilation (NR, NiR and GS), residual nitrate (NO3-), soluble protein content, and yield of spinach and lettuce plants were investigated under replacing 20% nitrate-N in the nutrient solution by L-glycine and blood meal amino acids. Seeds of the mention...

1. The effect of nitrate reduction and assimilation on the CO(2)/O(2) quotient of gas exchange has been used as an index of the relative rates of carbon and nitrogen assimilation in Chlorella pyrenoidosa. Changes in over-all metabolism induced by starvation, high light intensity, and nitrogen deficiency have been studied in comparison with the metabolism of cells growing at light-limiting inten...

Plants take up inorganic nitrogen (N) primarily as nitrate but also to a lesser extent as ammonium. Nitrate is reduced to nitrite in the cytoplasm by the highly regulated enzyme nitrate reductase. Nitrite is transported into the chloroplast and further reduced by nitrite reductase to ammonium. Ammonium is assimilated into the amino acid pool by the coordinate action of Gln synthetase (GS) and G...