Tuberculosis (TB) is a zoonotic infectious disease common to humans and animals which has been caused by a rod shaped, acid fast bacterium, called Mycobacterium bovis. The rapid and sensitive detection is a great challenge for TB diagnosis. The virulent strains of Mycobacterium tuberculosis complex (MTBC) have 16 different regions of difference (RD) in their genome which encode some important a...

The purpose of this work was to develop a simple procedure for the creation of a specific biorecognition layer for histidine-tagged (His-tagged) molecules. Such a layer was prepared by the spontaneous fusion of vesicles containing readily available plain (DOPC) and iminodiacetic acid (DOGS-NTA) phospholipids on a silica surface resulting in the formation of an NTA-containing supported lipid bil...

Absence of gradients and Nernstian equilibrium stripping (AGNES) is an emerging electroanalytical technique designed to measure free metal ion concentration. The practical implementation of AGNES requires a critical selection of the deposition time, which can be drastically reduced if the contribution of the complexes is properly taken into account. The resin titration (RT) is a competition met...

Structural and mechanistic characterization of proteins by fluorescence resonance energy transfer (FRET)1,2 requires the ability to incorporate fluorescent probes at specific, defined sites.2 For proteins that do not contain cysteine residues, site-specific fluorescent labeling can be accomplished by use of site-directed mutagenesis to introduce a cysteine residue at the site of interest, follo...

Effect of different type of organic compounds (humic acid, oxalate, ethylenediaminetetraacetic acid, nitrilotriacetic acid, phenol) on the photocatalytic removal of cyanide with TiO2 or ZnO was studied in this work with variation of the solution pH, contact time, initial cyanide concentration and type of organic compounds. Photocatalytic oxidation efficiency of cyanide with TiO2 was greatly aff...

Article history: Received on: 09/12/2014 Revised on: 17/01/2015 Accepted on: 22/02/2015 Available online: 27/05/2015 Plant derived drugs have been a part of the evolution of human healthcare for thousands of years. The present study was carried out to evaluate the antioxidant potential of ethanolic extract of Tabernaemontana divaricata on DEN initiated and Fe-NTA promoted renal damage in rats. ...

Immune Responses J. Bazzill, Y. Fan, E. Giang, S. Castillo, M. Law, J. J. Moon University of Michigan, Scripps Research Institute Purpose Subunit vaccines are highly attractive for vaccination against virulent pathogens due to their ease of manufacturing and proven safety. However, conventional subunit antigens are susceptible to degradation in vivo, and they lack the ability to activate B-cell...