A total of 179 urine samples were collected from patients suffering urinary tract infections admitted and visit Al-Hilla General Teaching Hospital in City, during a period April 2021 to December 2021, both sex (male female). Out 179,123 (68.7%) positive culture, whereas 56 (31.3%) showed no bacterial growth, To confirm the identification E. coli by use selective media (EMB agar medium, biochemi...