Tephrosia apollinea is a perennial shrublet widely distributed in Africa and is known to have medicinal properties. The current study describes the bio-assay (cytotoxicity) guided isolation of (-)-pseudosemiglabrin from the aerial parts of T. apollinea. The structural and stereochemical features have been described using spectral and x-ray crystallographic techniques. The cytotoxicity of isolat...

A double-label immunofluorescence technique was used to determine whether all normal fibroblast-like cells cultured from porcine connective tissues simultaneously express both type I collagen and fibronectin (FN). Cells surrounding explants in primary cultures from gingival and periodontal ligament (PL) tissue were heterogeneous in the expression of collagen and FN. Mass population cells at fir...

In this study we have examined the mechanism underlying the contact-mediated transfer of a lysosomal enzyme from lymphocytes to fibroblasts in culture. We found that although antibody against the mannose 6-phosphate lysosomal targetting receptor (MPR) completely inhibited fibroblast uptake of the lysosomal enzyme beta-glucuronidase (Gus) from the culture medium, it had no effect on the transfer...

study these questions, in vitro cell lines with typical cytogenetic abnormalities, such as the Philadelphia chromosome, would be useful. So far, research has been hampered by lack of such cell lines derived from bone marrow. Hentel and Hirschhorn’ reported that a proportion of bone marrow-derived fibroblasts had karyotypic abnormalities identical with those in mitoses observed in the bone marro...

Rationale: Inflammation, increased fibroblast proliferation, and increased deposition of extracellular matrix (ECM) are hallmarks of early lung fibrosis and asthma. Transforming growth factor(TGF) has been suggested as a key regulator of lung tissue homeostasis with several and often opposite effects on fibroblast proliferation and ECM production. In human and animal model systems, it has been ...

The in vitro generation of memory cells reactive to transplantation antigens is described. Blast cells, obtained from rat lymphocytes sensitized on xenogeneic or allogeneic fibroblast monolayers, reverted to secondary small lymphocytes after transfer from the foreign sensitizing to syngeneic monolayers. These secondary small lymphocytes had a limited in vitro life span of 4-6 wk. They manifeste...

In an effort to elucidate control mechanisms for developmentally programmed cell death, conditions were sought which rescue the cells destined to die. Three areas of mesodermal cell death in the chick wing were examined, the posterior necrotic zone (PNZ) , the opaque patch (OP) and apical mesoderm. The PNZ and OP are areas of normally programmed cell death whereas the apical mesoderm undergoes ...

We have shown previously that phagocytosis of cells dying by apoptosis results in transfer of whole or fragments of chromosomes into the nucleus of the recipient cell. Although DNA transfer was detected in normal cells, stable propagation of the transferred DNA was only observed in cells deficient in p53. Here we show that mouse embryonic fibroblast cells lacking the p21 (Cip1/Waf1) cyclin-kina...

By using a semi-quantitative immunoblotting technique, we have analyzed serum immunoglobulin G (IgG) reactivities of patients with limited cutaneous systemic sclerosis and anticentromere antibodies, patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies, patients with diffuse systemic sclerosis without antitopoisomerase 1 or anticentromere antibodies and age- and gender-mat...

We report a sibship of a sister and brother with osteoporosis-pseudoglioma syndrome. Several other family members became blind or showed signs of bone involvement. There was considerable consanguinity in the pedigree. The proband was small in size and had prominent skeletal deformities and clinical muscle weakness. These features were not present in her brother, suggesting clinical variability....