BACKDROUND Ovarian carcinoma is a leading cause of death in gynecological malignancy. Ovarian surface epithelial serous and mucinous tumours are classified as benign, borderline, and malignant. The identification of borderline tumours most likely to act aggressively remains an important clinical issue. AIM This work aimed to study DNA ploidy and nuclear area in ovarian serous and mucinous; be...

By identifying aggressive cutaneous squamous cell carcinoma (cSCC) in patients who are at high risk for recurrences or second primaries after resection, intensive surveillance and therapy may decrease morbidity and mortality. We investigated the role of nuclear morphometry (karyometry) in differentiating between aggressive and nonaggressive cSCC. We retrospectively analyzed cSCC lesions from 40...

Breast cancer is the most prevalent cancer and the main cause of cancer-related death in women worldwide. There are limitations associated with the existing clinical tools for breast cancer detection and alternative modalities for early detection and classification of breast cancer are urgently needed. Here we describe an optical imaging technique, called multispectral diffuse optical tomograph...

Haemic neoplasia (HN) in bivalves has been reported in association with mass mortality events in various species of molluscs. The aim of this work was to quantify the nuclear morphometry and DNA content of neoplastic cells of mussels Mytilus galloprovincialis affected by HN using nuclear densitometry in Feulgen-stained preparations. The results were also compared with a population of normal mus...

Despite interest in the use of nuclear morphometry for cancer diagnosis and prognosis as well as to monitor changes in cancer risk, no generally accepted statistical method has emerged for the analysis of these data. To evaluate different statistical approaches, Feulgen-stained nuclei from a human lung epithelial cell line, BEAS-2B, and a human lung adenocarcinoma (non-small cell) cancer cell l...

Buffalofishes (Genus Ictiobus) are large, robust-bodied suckers adapted to large rivers and lakes of North America. Currently recognized species are readily diagnosed by morphological characters, and the group is known from fossils dating back to the Miocene. However, sympatrically occurring species in the Mississippi River Basin are known to hybridize in nature and in the laboratory. Here we d...

The ImageStream system combines the capabilities of microscopy and flow cytometry in a single platform for quantitative image-based cellular assays in large and heterogeneous cell populations. Here we demonstrate the simultaneous measurement of druginduced apoptosis and NF-kB nuclear translocation using a combination of intensity-based and morphometric parameters. Apoptotic and necrotic cells a...

BACKGROUND Oral cancer is revisited on a pathologist perspective with advanced imaging technique. OBJECTIVE The present study assessed the new malignancy grading system at tumor proper (TP) and Bryne's grading system at invasive tumor front (ITF), morphometric features using software, to clarify their associations with prognosis of oral cancers. METHODS Histologically confirmed oral squamou...