نتایج جستجو برای: nucleated cell
تعداد نتایج: 1685735 فیلتر نتایج به سال:
The relationship between the crack nucleation and stress-induced martensitic transformation in the retained massive austenites (RM’s) of austempered ductile irons (ADIs) was examined in detail by carrying out tensile tests and scanning electron microscope (SEM) observations for an ADI material. The SEM observations revealed that cracks were not nucleated in the peripheral regions of graphite no...
Three different closed procedures for concentrating bone marrow progenitor cells prior to cryopreservation have been compared. These were by a manual double centrifugation method, a Hemonetics 30 cell separator and an Aminco Celltrifuge. The best results were achieved using the paediatric pheresis set on the Hemonetics model 30. Marrow was frozen in 120 ml aliquots in a programmed freezer with ...
A modified method was developed for measuring the frequency of variant erythrocytes at the glycophorin A locus using a single beam cell sorter (SBS). Fluoresceinor phycoerythrin-labeled monoclonal antibod ies specific for the M or N glycophorin A alÃ-eleswere used for the SBS assay. To prevent contamination of nucleated cells in the sorting windows, the nucleated cells in the fixed erythrocyte ...
Biomimetic postcapillary expansions for enhancing rare blood cell separation on a microfluidic chip.
Blood cells naturally auto-segregate in postcapillary venules, with the erythrocytes (red blood cells, RBCs) aggregating near the axis of flow and the nucleated cells (NCs)--which include leukocytes, progenitor cells and, in cancer patients, circulating tumor cells--marginating toward the vessel wall. We have used this principle to design a microfluidic device that extracts nucleated cells (NCs...
We evaluated demographic characteristics and graft composition as risk factors for acute graft-versus-host disease (GVHD) in 160 adult recipients of HLA-identical allogeneic blood stem cell transplants. The patients received a median nucleated cell dose of 7.9 3 108/kg and median C341 cell dose of 5.6 3 106/kg. GVHD prophylaxis consisted of cyclosporine (CSA) and steroids, tacrolimus (FK506) an...
umbilical cord blood (ucb) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (hscs). because of insufficient number of cord blood cd34+ cells, the expansion of these cells seems to be important for clinical application. mesenchymal stromal cells (mscs), playing an important role in hscs maintenance, were used as feeder layer. apo...
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