The most common direct detection methods are isolation or in-vitro cultivation, electron microscopy, immunofluorescence, immunohistochemistry, antigen enzyme-linked immunosorbent assay (antigen-ELISA), nucleic-acid hybridisation (NAH), macroand microarrays and the various techniques of nucleic acid amplification, such as the polymerase chain reaction (PCR) or the isothermal amplification method...

Nucleic acid three-way junctions (3WJs) play key roles in biological processes such as nucleic acid replication in addition to being implicated as dynamic transient intermediates in trinucleotide repeat sequences. Structural modulation of specific nucleic acid junctions could allow for control of biological processes and disease states at the nucleic acid level. Trinucleotide repeat expansions ...

The assessment of nucleic acid purity by the A260/A280 ratio has been used for a number of years. This measurement, however, does not provide all the information that investigator needs to assess nucleic acid preparations. The detection of impurities common to nucleic acid preparations that are not detected with absorbance measurements at 260 nm and 280 nm can in most instances be accomplished ...

The promise of accessing tissues beyond the liver is driving deals between pioneers in field nucleic-acid-based drugs and companies developing delivery platforms.

The highly specific feulgen reaction for demonstrating desoxypentose nucleic acid has proved to be of unequal utility in the hands of bacterial cytologists for the study of the bacterial nucleus (Milovidov, 1935), and at present the principal cytochemical evidence is derived from basic staining following hydrochloric acid hydrolysis or following ribonuclease digestion. It has been generally acc...

The characteristic serologic feature of systemic lupus erythematosus (SLE) is autoantibodies against one's own nucleic acid or nucleic acid-binding proteins - DNA and RNA-binding nuclear proteins. Circulating autoantibodies can deposit in the tissue, causing inflammation and production of cytokines such as type 1 interferon (IFN). Investigations in human patients and animal models have implicat...