This study represents phylogenetic analyses of two woody polygonaceous genera Calligonum and Pteropyrum using both chloroplast fragment (trnL-F) and the nuclear ribosomal internal transcribed spacer (nrDNA ITS) sequence data. All inferred phylogenies using parsimony and Bayesian methods showed that Calligonum and Pteropyrum are both monophyletic and closely related taxa. They have no affinity w...

Single-nucleotide polymorphisms are a type of genetic variation that may be utilized to investigate illness vulnerability. Recurrent abortion is linked hereditary thrombophilias, which include various disorders such as the MTHFR thermolabile variation. No link known between these two mutations and risk recurrent spontaneous abortion. It unknown if pregnant women with at losing their baby. Thirt...

A 50-day feeding trial was conducted to evaluate the effects of yeast nucleotide in diets on growth performance, serum immune indices and muscle composition of Ancherythroculter nigrocauda (mean initial body weight, 23.30±0.59 g). Seven isonitrogenous (approximately 42.76% crude protein) and isoenergetic (17.43 KJ g−1 gross energy) experimental diets with varying levels of yeast nucleotide (0[c...

Pentamidine (PEN) is an alternative compound to treat antimony-resistant leishmaniasis patients, which cellular target remains unclear. One approach to the identification of prospective targets is to identify genes able to mediate PEN resistance following overexpression. Starting from a genomic library of transfected parasites bearing a multicopy episomal cosmid vector containing wild-type Leis...

A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7T, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence analysis showed that BII-R7T represented a member of the genus Stenotrophomonas (class Gammaproteobacteria), and was related most closely to Stenotrophomonas...

Detailed structural and biochemical studies with the human cytomegalovirus (HCMV UL54) DNA polymerase are hampered by difficulties to obtain this enzyme in large quantities. The crystal structure of the related RB69 DNA polymerase (gp43) is often used as a model system to explain mechanisms of inhibition of DNA synthesis and drug resistance. However, here we demonstrate that gp43 is approximate...