Outer Membrane Vesicles (OMVs) of Gram-negative bacteria are spherical membrane-enclosed entities of endocytic origin. Reported in the consortia of different bacterial species, production of OMVs into extracellular milieu seems essential for their survival. Enriched with bioactive proteins, toxins, and virulence factors, OMVs play a critical role in the bacteria-bacteria and bacteria-host inter...

Outer membrane vesicles (OMVs) produced by Gram-negative bacteria provide an interesting research material for defining cell-envelope proteins without experimental cell disruption. OMVs are also promising immunogenic platforms and may play important roles in bacterial survival and pathogenesis. We used in-solution trypsin digestion coupled to mass spectrometry to identify 90 proteins present in...

To the Editor—We read with great interest the article by Jones et al. [1] describing the dynamics of carriage of Neisseria meningitidis among military recruits. Of particular interest was their finding of serosubtype-specific bactericidal responses to the acquired meningococcal strains, a finding that supported previous results from various laboratories on the role of PorA antigens in eliciting...

Outer membrane vesicles (OMVs) shed from bacteria contribute to pathogenesis by promoting colonization of host tissues and trafficking virulence factors into host cells via fusion with the host cell plasma membrane. Glyeraldehyde-3-phosphate dehydrogenase (GAPDH) is also secreted by prokaryotes, but enhances pathogenesis by promoting adhesion of bacteria to host cell surfaces. However, GAPDH is...

Many Gram-negative bacterial species release outer membrane vesicles (OMVs) that interact with the host by delivering virulence factors. Here, we report for the first time that RNA is among the wide variety of bacterial components that are associated with OMVs. To characterize the RNA profiles of bacterial OMVs, we performed RNA deep sequencing analysis using OMV samples isolated from a wild ty...