Optical tweezers use focused laser light to manipulate microscopic particles. We discuss the underlying physics of the technique in terms of a gradient force exerted by the light on the particles. The versatility of optical tweezers is highlighted, in particular, we explain how spatial light modulators and various imaging methods have greatly enhanced their range of applications.

Optical branch waveguides are one of the most important optical elements and have been widely exploited for optical communication systems. However, prevailing devices are typically solid and have limit in tunability. Liquid optical devices have attracted more interest for the advantage of tunability of liquid media, but their signals suffer serious leakage if the refractive index (RI) of liquid...

This paper is devoted to new optical methods, which are supposed to be used for liveness detection on fingers. First we describe the basics about fake finger use in fingerprint recognition process and the possibilities of liveness detection. Then we continue with introducing three new liveness detection methods, which we developed and tested in the scope of our research activities--the first on...

Optical traps have shown to be a flexible and powerful tool for 3D manipulations on the microscale. However, when it comes to sensitive measurements of particle displacements and forces thorough calibration procedures are required, which can be already demanding for trapped spheres. For asymmetric structures, with more complicated shapes, such as helical bacteria, novel calibration schemes need...

Imaging non-adherent cells by super-resolution far-field fluorescence microscopy is currently not possible because of their rapid movement while in suspension. Holographic optical tweezers (HOTs) enable the ability to freely control the number and position of optical traps, thus facilitating the unrestricted manipulation of cells in a volume around the focal plane. Here we show that immobilizin...

The statistical properties of speckles in paraxial optical systems depend on the system parameters. In particular, the speckle orientation and the lateral dependence (x and y) of the longitudinal speckle size can vary significantly. For example, the off-axis longitudinal correlation length remains equal to the on-axis size for speckles in a Fourier transform system, while it decreases dramatica...

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Recently reported metamaterial analogues of electromagnetically induced transparency enable a unique route to endow classical optical structures with aspects of quantum optical systems. This method opens up many fascinating prospects on novel optical components, such as slow light units, highly sensitive sensors and nonlinear devices. In particular, optical control of electromagnetically induce...

Computer-generated holograms displayed by phase-modulating spatial light modulators have become a well-established tool for beam shaping purposes in holographic optical tweezers. Still, the generation of light intensity patterns with high spatial symmetry and simultaneously without interfering ghost traps is a challenge. We have implemented an iterative Fourier transform algorithm that is capab...

Huygens’ principle can be used to determine various experimentally verifiable and predictable behavior of the path of light through any optical system. However, as seen in the chapter on Light, the Huygens wavefront construction can be become complicated, especially in systems with a large number of optical components. A simpler approach to track the behavior of light is based on the propagatio...