The carbazole 1,9a-dioxygenase (CARDO) system of Pseudomonas resinovorans strain CA10 consists of terminal oxygenase (CarAa), ferredoxin (CarAc), and ferredoxin reductase (CarAd). Each component of CARDO was expressed in Escherichia coli strain BL21(DE3) as a native form (CarAa) or a His-tagged form (CarAc and CarAd) and was purified to apparent homogeneity. CarAa was found to be trimeric and t...

Hydroxyapatite Ca10(PO4)6(OH)2 (HAp) is an important bioactive material for bone tissue reconstruction, due to its highly thermodynamic stability at a physiological pH without bio-resorption. In the present study, Ag:HAp and corresponding + D3 thin films (~200 nm) coating were obtained by vacuum deposition method on Ti substrate. The samples exposed different UV irradiation times, in order inve...

Coronary atherosclerosis is frequently associated with calcification of arterial plaque. To understand the mechanisms responsible for the formation of atherosclerotic calcification, we examined human coronary arteries for the presence and extent of mineral. In sections stained specifically for mineral, staining was diffuse and present in all atherosclerotic plaques. Hydroxyapatite was not detec...

Hydroxyapatite (Ca10(PO4)6(OH)2, HAp) and dicalcium phosphate anhydrous (Ca(H2PO4)2, DCPA) were coated onto titanium substrates using the thermal substrate method in an aqueous solution containing calcium and phosphate ions at 150◦C with pH values in the range 4–8. Specimens with the HAp and DCPA layer were immersed in a simulated body fluid (SBF) to examine the dissolution and induced HAp grow...

In numerous biominerals exposed to ionizing radiation some stable paramagnetic species are induced. Their nature can differ depending on chemical composition of biominerals. The method of choice to study paramagnetic centers in biominerals is electron paramagnetic resonance (EPR) spectroscopy. When the relationship between EPR signal intensity of stable paramagnetic center and the dose has a li...

Ideally, bioactive ceramics for use in alveolar ridge augmentation should possess the ability to activate bone formation and, thus, cause the differentiation of osteoprogenitor cells into osteoblasts at their surfaces. Therefore, in order to evaluate the osteogenic potential of novel bone substitute materials, it is important to examine their effect on osteoblastic differentiation. This study e...

Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth. In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method....

Fluorapatite, Ca10(PO4)6F2. is a widely spread form of calcium phosphate present particularly in biological material. Human hard tissues contain crystals structurally related to apatite. Fluoride can be found in various natural sources and is also used for its beneficial action in caries prevention. Fluorapatite belongs to the spatial group P6(3/m) (C(6h)2) and consists of 3 ions: F-, Ca2+, PO4...