The development of phage P22 following infection involves a mandatory recombination step, the timing of which is the same in both the lytic and lysogenic pathways. Covalently circular molecules of phage DNA were identified by alkaline sucrose sedimentation of infected cell lysates. The time of appearance of these structures corresponds to the time of the essential recombination step. On infecti...

A smooth colony strain, resistant to phages lambda and P22, was isolated from sewage and identified as Escherichia coli (strain H). Four temperate phages plaquing on strain H were isolated from sewage. The archetype, HK620, does not plaque on strains C and K12 of E. coli nor on the LT2 strain of Salmonella enterica. Bacterial mutants resistant to a clear plaque mutant of HK620 produce rough col...

P22-mediated generalized transduction in S. typhimwiwn occurs in both sensitive and lysogenic recipient bacteria, but the efficiency of transduction is significantly higherinsensitivecells t.han in wild-type P22 lysogens. This reduction in the efficiency of transduction is under the control of the prophage sieA gene; this gene is also responsible for the exclusion of superinfect,ing phage. Expe...

it has been estimated that more than 1.7 million individuals suffer a traumatic brain injury (tbi) each year. tbi causes secondary biochemical and metabolic changes which contribute to subsequent tissue damage and associated neuronal cell death. two early and delayed events occur after tbi, which results in neurological deficits. primary injury events include the mechanical damage that occur at...

GENETIC AND STRUCTURAL ANAYLYSIS OF P22 LYSOZYME P22 lysozyme , encoded by gene 19, is an essential phage protein responsible for hydrolyzing the bacterial cell wall during lytic infection. P22 lysozyme is related to T4 lysozyme in its mode of action, substrate specificities , and in its structure. Gene 19 was located on the phage genome , subcloned and then sequenced. Lysozyme was produced in ...

Rifampin, in addition to interfering with intracellular growth of the ribonucleic acid-containing phage MS2, also inhibits the release of mature phage particles from Escherichia coli cells.

The Bacillus cereus group bacteriophage TsarBomba, a double-stranded DNA Myoviridae, was isolated from soil collected in Saratov, Russia. TsarBomba was found to be similar to Bacillus phages BCP78 and BCU4, and to have a wide host range among Bacillus cereus group species.

The Italian contribution to the Socialist Economic Calculation Debate (SECD) cannot be limited important and fundamental works by Pareto Barone. In fact, if their contributions are still ambiguous we have wait for Mises’ paper in 1920 order get needed clarifications, during 1960s Bruno Leoni follows Austrian footsteps on topic, stimulating Italy a great debate. Leoni’s role is because of debate...

We consider an eigenvalue problem for a system in [0, 1]:        0 1 1 0 d dx + p11(x) p12(x) p21(x) p22(x) ϕ (1) (x) ϕ (2) (x) = λ ϕ (1) (x) ϕ (2) (x) ϕ (2) (0) cosh µ − ϕ (1) (0) sinh µ = ϕ (2) (1) cosh ν + ϕ (1) (1) sinh ν = 0 with constants µ, ν ∈ C. Under the assumption that p21, p22 are known, we prove a uniqueness theorem and provide a reconstruction formula for p11 and p12 from t...

Infection of Salmonella enterica with lytic mutants of either P22 or SE1 bacteriophages triggers the expression of its DNA damage-inducible SOS response through a lexA-dependent pathway. This induction of the SOS system strictly requires the presence of the bacteriophage kil gene. Accordingly, plasmid over-expression of the kil gene also promotes the S. enterica SOS network induction. Furthermo...