The influence of homopolymer architecture on the properties polyion complex micelles is reported. Using a combination dynamic and static light scattering, authors show how only relevant in kinetically trapped states formed by electrostatic assembly poly(N-isopropyl acrylamide-block-styrene sulfonate) (p(NIPAM-b-SS) linear, 4-arm, 8-arm star quaternized poly(dimethyl amino ethyl acrylate) (PDMAE...

periphery, which forms a closed membrane-like structure [3]. When a critical stage is reached dendrimers cannot grow because of steric hindrance. This is sometimes referred to as ‘starburst effect’. In case of PAMAM dendrimers the starburst effect can be observed after the tenth generation [4]. Number of terminal functional groups also increases exponentially with the increased branch density. ...

H NMR spectra to support data for Table 1 and Figure 1: For synthesis of hydrophobically modified dendrimers, dodecyl chloroformate was adjusted to 5, 10 and 20 mol % of G5OH PAMAM dendrimer hydroxyl groups, and the amount of cholesteryl chloroformate was 0.86, 2, 5, and 20 mol % of dendrimer hydroxyl groups. The corresponding modified dendrimers were designated as D1, D2, D3 and C1, C2, C3, C4...

Interior tertiary amine groups of PAMAM-OH dendrimers (hydroxyl-terminated polyamidoamine, PAMAM) were modified by methylation to make these polymers have a more cationic character, which enabled electrostatic interaction between PAMAM-OH and plasmid DNA. A methylation reaction was dose-dependent, producing internally quaternized PAMAM-OH (QPAMAM-OH), thereby making tertiary amine/quaternary am...

Starburst polyamidoamine (PAMAM) dendrimers are a new type of synthetic polymer characterized by a branched spherical shape and a high density surface charge. We have investigated the ability of these dendrimers to function as an effective delivery system for antisense oligonucleotides and 'antisense expression plasmids' for the targeted modulation of gene expression. Dendrimers bind to various...

Experimental data indicates that poly(amidoamine) (PAMAM) dendrimers flatten when in contact with a substrate, i.e. they are no longer spherical, but resemble flat disks. In order to better understand the deformation behavior of these branched polymers, a series of atomistic molecular dynamics simulations is performed. The resulting flattened dendrimer conformations are compared to atomic force...

Dendrimers have been widely used as nanostructured carriers for guest species in a variety of applications in medicine, catalysis, and environmental remediation. Theory and simulation methods are an important complement to experimental approaches that are designed to develop a fundamental understanding about how dendrimers interact with guest molecules. This review focuses on computational stud...

Objectives. MicroRNAs (miRNAs) are involved in the regulation of most biological processes and also contribute to many types of disease. Fibroblast cells, such as MRC-5, are often used in biological researches utilizing cell transfection methods due to their difficult to transfect nature. Cells can be genetically engineered by using viral and non-viral methods. Poly(amidoamine) (PAMAM) dendrime...