Background: An endemic disease cutaneous leishmaniasis CL was spread in Iraq. In 2020, there were more cases than previous years, with most occurring rural areas. this scenario, the current diagnostic technique must be replaced a sensitive one. This research aimed to examine if polymerase chain reaction (PCR) is an efficient approach for identifying leishmanial parasites skin biopsies from Iraq...

Abstract Koi herpesvirus (KHV) has been responsible for worldwide KHV disease (KHVD) outbreaks, resulting in high mortality rates, and reported Cyprinus carpio cultivation. A Triplex Polymerase Chain Reaction (T-PCR) was developed the detection of gene to improve diagnosis infection. Three sets primers were designed targeting specific sequences which frequently used PCR assay detecting (modific...

AIMS To develop a multiplex polymerase chain reaction (PCR) method to facilitate identification of mycobacterial isolates. METHODS Type strains of 14 species of mycobacteria and 56 clinical isolates were lysed by boiling in TE Triton. The lysate (5 microliters) was used directly in a PCR reaction incorporating three pairs of PCR primers expected to amplify fragments from the genome of (a) all...

Abbreviations: ARP: acidic ribosomal protein CHS: chalcone synthase DDRT-PCR: differential display reverse transcriptase polymerase chain reaction DETs: differentially expressed transcripts DPA: days post anthesis ESTs: expressed sequence tags MBD: methyl CpG binding domain OPT: oligopeptide transporter protein PCR: polymerase chain reaction PG: polygalacturonase enzyme RRF: ribosome recycling ...

A simple method for viral DNA extraction using chelex resin was developed. The method used was eco-friendly and cost effective compared to other methods such as phenol chloroform method which use health hazardous organic reagents. Further, a polymerase chain reaction (PCR) based detection of canine parvovirus (CPV) using primers from conserved region of VP2 gene was developed. To increase the s...

Background. Persistent Escherichia coli asymptomatic bacteriuria (ASB) is common among persons with diabetes mellitus, but the duration of colonization and the rates of recolonization are unknown. We estimated the duration of colonization and the rate of recolonization among successively isolated E. coli from diabetic women with ASB and compared the virulence profiles with uropathogenic and com...

BACKGROUND Estimates of Human Papillomavirus (HPV) prevalence in a population prior to and after HPV vaccine introduction are essential to evaluate the short-term impact of vaccination. METHODS Between 2010 and 2012 we conducted a population-based cross-sectional study in Germany to determine HPV prevalence, genotype distribution and risk factors for HPV-infection in women aged 20-25 years. W...

A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (...

ObjectivesUndetectable or low-level hepatitis B virus (HBV) DNA and drug resistance mutations in patients may increase the risk of HBV transmission cause active viral replication other clinical problems. Here, we established a highly sensitive practical method for detection using polymerase chain reaction (PCR) -based CRISPR-Cas13a system (referred to as PCR-CRISPR) evaluated its capability sam...