A technique is described for embedding tissue culture cells that have been adsorbed or grown on Millipore filters. The acetone used during the embedding process rendered the filters transparent so that specific areas or cells could be chosen with the aid of the light microscope. Lymphoblastoid cells processed on the filters possessed well-defined plasma membranes and microvilli which were rarel...

We assessed the effects of arbutin on the pigmentation of cultured normal human melanocytes. As indicated by a cell-blotting assay, arbutin at concentrations in the range of 0.5-8 mM increased the pigmentation of the cultured melanocytes, while kojic acid at concentrations in the range of 0.5-4 mM decreased the pigmentation. The pigmentation-augmenting effect of arbutin was further confirmed by...

Pelleting inactivates SODle viruses and aggregates other dispersed :material. Methods for separation should be developed in which desired particles are always kept in suspension. Rotors that cODlbine continuous-flow centrifugation with isop"ycnic banding can achieve this effect. In our SySteDl a liquid density gradient is set up in a spinning rotor next to the wall in each of 4 sector-shaped cO...

Pelleting costs money, and better pellets cost a little more. Payback comes in the form of convenience in handling the feed and more efficient use of the feed by the animal. Improved efficiency is due in part to heat processing which reduces pathogens and makes starches more digestible. A significant portion of the improvement is related to the physical form of the pellet. Durable pellets reduc...

A rapid method of ultracentrifugation pelleting of avian adenovirus (AAV) from small volume of chloroform treated infected cell culture fluid or allantoic fluid was adapted for isolation of adenoviral DNA. The viral DNA extracted from semipurified viruses was found to be intact on agarose gel and pure enough (A260/280 = 1.85-1.92) for restriction enzyme analysis. Restriction endonuclease analys...

Tujuan penelitian ini untuk mengetahui filler, binder dan inokulan yang dapat digunakan sebagai bahan pelleting benih padi gogo varietas Inpago 13 Fortiz. Penelitian menggunakan Rancangan Acak Kelompok (RAK) diulang sebanyak tiga kali. Perlakuan terdiri dari (1) Tanpa pellet; (2) Tanah + CMC ; (3) Mikoriza; (4) Trichoderma; (5) Dolomit (6) Asam Humat (7) AG; (8)Tanah AG (9) (10) Dolomit; (11) H...

This paper presents theoretical and experimental research studying the influence of process parameters on quality biomass pellets. A validated mathematical model was developed, expressing density pellets as determined by moisture content, compression pressure, heat, initial material, pelleting speed volume material. The experiments for determining these into optimizing were conducted a heated s...