High performance liquid chromatography (HPLC) is increasingly being used to estimate variant haemoglobins. A case of haemoglobin S/D (HbS/D) is presented, which was misdiagnosed as haemoglobin S/E (HbS/E) by HPLC. The patient was a 22 year old woman with sickle cell anaemia. Subsequent haemodilution by blood transfusion clearly elucidated the haemoglobin D peak on HPLC. Sample dilution experime...

High performance liquid chromatography (HPLC) is increasingly being used to estimate variant haemoglobins. A case of haemoglobin S/D (HbS/D) is presented, which was misdiagnosed as haemoglobin S/E (HbS/E) by HPLC. The patient was a 22 year old woman with sickle cell anaemia. Subsequent haemodilution by blood transfusion clearly elucidated the haemoglobin D peak on HPLC. Sample dilution experime...

A neurophysiological detector (NPD) is a hybridization of olfactory system neurons of the fish crucian carp, Carassius carassius L., with a computerized electronic device connected to a high-performance liquid chromatograph (HPLC). This system makes it possible to measure neurophysiological activities in the olfactory system of C. carassius L. after exposure of this fish to alarm pheromones. Th...

Ultrafast reversed-phase high-performance liquid chromatographic (HPLC) separations are often needed for analyses related to combinatorial chemistry, studies in liquid chromatography-mass spectrometry, and other applications in which very rapid sample turnaround is paramount. Unfortunately, no consensus exists regarding the best column technology for optimally performing the desired rapid separ...

in this project, eggshell membrane was used as a solid phase extraction sorbent for extraction of morphine followed by hplc analysis. at first, raw eggshell membrane was used and then, because of low adsorption of morphine on untreated eggshell membrane (

silymarin is a mixture of flavonolignans from the seeds of silybum marianum (l.) gaertner,containing silybinin, isosilybinin, silydianin, silychristin and the dihydroflavonol of taxifolin. flavonolignancomponents are largely responsible for the medical benefits attributed to silymarin. the aim of this researchwas to study the variations in composition and content of flavonolignans of silymarin ...

the use of accelerated temperature stability studies using the arrhenius relationship is routine for estimating the stability of a drug at room temperature. therefore, the acceleration method is used to determine the expiration date of dexamethasone aqueous formulation manufactured in iran. a high-performance liquid chromatography (hplc) method utilizing ultraviolet (uv) detection for dexametha...

Within the framework of this study, a quantitative HPLC-MS/MS (high-performance liquid chromatography with mass spectrometric detection) method for determination vancomycin was developed. The in human plasma validated using chromato-mass method. accordance requirements EAEU bioanalytical methods. analytical range thetechnique 1–100 μg/mL plasma.

Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons fo...

The use of high-performance liquid chromatography (HPLC) to measure ribavirin in serum and other biological fluids has been limited by endogenous interfering substances. We report an HPLC procedure based on the extraction of ribavirin from serum, plasma, or cerebrospinal fluid with a boronate affinity gel, which uses a 3-methylcytidine internal standard. This assay is sensitive (to 0.4 microM),...