نتایج جستجو برای: phage library

تعداد نتایج: 138241  

Journal: :Molecular cancer research : MCR 2010
Bing Du Honghui Han Ziqiang Wang Lisha Kuang Lei Wang Liping Yu Miao Wu Zhongliang Zhou Min Qian

Hepatocellular carcinoma is one of the deadliest cancers in the world. In this study, a hepatocarcinoma-specific binding peptide, which could be used for drug delivery in targeting therapy, was obtained by in vivo phage display technology. After three rounds of panning, only the potential motif Pro-Ser was found in 80 sequenced phage clones. Phage A54 (sequence AGKGTPSLETTP) was shown to be the...

Journal: :Acta biochimica et biophysica Sinica 2007
Liyan SUN Taiwei CHU Yi WANG Xiangyun WANG

A dodecapeptide EDIKPKTSLAFR ligand targeting CEN-1 human nasopharyngeal carcinoma (NPC) was identified by in vivo phage display. Two tridecapeptides and their derivatives, named YR13 (YEDIKPKTSLAFR), EY13 (EDIKPKTSLAFRY), EY13-NH2 (EDIKPKTSLAFRY-NH2) and Fmoc-YR13 (Fmoc-YEDIKPKTSLAFR), were synthesized and radiolabeled with 131I. The stability in vitro, biodistribution and tissue distribution ...

Journal: :Protein engineering 1998
M Hirose T Hayano H Shirai H Nakamura M Kikuchi

We have isolated anti-glutathione antibodies from a human synthetic phage antibody scFv library (Nissim,A., Hoogenboom,H.R., Tomlinson,I.M., Flynn,G., Midgley,C., Lane,D. and Winter,G., 1994, EMBO J., 13, 692-698). Glutathione (GSH) conjugates with carrier proteins, such as bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH) and human lysozyme (LZM), were used as antigens. After four cy...

Journal: :Science 1996
T N Schumacher L M Mayr D L Minor M A Milhollen M W Burgess P S Kim

Genetically encoded libraries of peptides and oligonucleotides are well suited for the identification of ligands for many macromolecules. A major drawback of these techniques is that the resultant ligands are subject to degradation by naturally occurring enzymes. Here, a method is described that uses a biologically encoded library for the identification of D-peptide ligands, which should be res...

Journal: : 2023

Antimicrobial resistance is considered by WHO as one of the most important threats to public health in twenty-first century. According forecasts, 2025, many first-line antimicrobials will lose their effectiveness and «post-antibiotic era» begin. Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. representatives ...

2016
Daisuke Ando Masaki Inoue Haruhiko Kamada Shintaro Taki Takeshi Furuya Yasuhiro Abe Kazuya Nagano Yasuo Tsutsumi Shin-ichi Tsunoda

Tumor necrosis factor-α (TNF), which is an immuno-modulatory cytokine, has been suggested to cause inflammatory responses as well as protection against tissue dysfunction by binding two types of TNF receptor (TNFR1/TNFR2). However, the physiological effects of TNFR2-specific activation remain unclear. We therefore aimed to generate a TNF mutant with full TNFR2-selective agonist activity as a fu...

Journal: :Nucleic acids research 1982
L M Kunkel U Tantravahi M Eisenhard S A Latt

Fluorescence activated sorting of chromosomes from 49,XXXXY human lymphoblasts has been used to obtain DNA enriched for the human X. This DNA was cloned in lambda phage Charon 21A to obtain a library of approximately 60,000 pfu. Phage inserts free of human highly repeated DNA sequences are localized to different regions of the human X by two independent hybridization analyses. The first utilize...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 1999
J M Kupsch N H Tidman N V Kang H Truman S Hamilton N Patel J A Newton Bishop I M Leigh J S Crowe

A human single-chain Fv (scFv) library as fusion to phage was constructed from donors with a high titer of autoantibodies. The library was subjected to three rounds of positive selection on human melanoma cells and negative selection on human peripheral blood mononuclear cells. Two scFv clones, B3 and B4, were isolated that bound melanoma cells in cell ELISA and fluorescence-activated cell sort...

Journal: :BioTechniques 2000
M L Phipps X Xu S Nock P D Kassner

Filamentous phage display has become a powerful method for the discovery of affinity-binding reagents (6). Linear and constrained peptides, antibody fragments (scFvs and Fabs) and some alternative binding domains have all been displayed on phage particles by fusing to one of the phage coat proteins. Although several phage proteins (derived from gVIII, gVI, gVII and gIX) have all been used as fu...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید