نتایج جستجو برای: phage typing
تعداد نتایج: 40577 فیلتر نتایج به سال:
Fluorescent amplified fragment length polymorphism (AFLP) was applied to 46 Salmonella enterica serovar Typhimurium isolates of Australian origin comprising nine phage types, by using the restriction enzymes MseI and EcoRI and all 16 possible MseI +1-EcoRI +1 primer pair combinations. AFLP in the present study showed a very good discrimination power with a Simpson index of diversity of 0.98, an...
The presently accepted method for marking individual strains of Staphylococcus aureus for epidemiological investigation is bacteriophage typing. However, phage typing is not a stable marker and many strains cannot be successfully typed. Serological typing is not readily available and preparation of sera is difficult. The aminopeptidase profile method described by Krawezyk and Huber was used to ...
In settings of high methicillin-resistant Staphylococcus aureus (MRSA) prevalence, detection of nosocomial transmission events can be difficult without strain typing. Prospective typing of all MRSA isolates could potentially identify transmission in a timely fashion, making infection control responses to outbreaks more effective. We describe the development and evaluation of a novel 19-target b...
The application of bacteriophages biocontrol requires the formulation genetically distinct in a phage cocktail. Random Amplified Polymorphic DNA (RAPD) - PCR is considered cheap, reproducible, and readily applicable tool detecting diversity compared to other molecular techniques such as whole-genome sequencing. We used this study RAPD-PCR technique assess genetic 28 infecting Pseudomonas aerugi...
In December 2000, an increase in the number of Salmonella enteritidis cases was reported in Ontario by the Ontario Central Public Health Laboratory. In January 2001, S. enteritidis isolates from Ontario for the months of November and December 2000, and January 2001, were submitted to the National Laboratory for Enteric Pathogens for phage typing. Several isolates of a rare phage type, PT30, wer...
Bacteriophages, viruses that infect bacteria, were discovered at the beginning of the twentieth century. Today, phages are being used as antibacterial agents, in phage display screening, in the development of phage delivery vaccines, as targeted gene therapy delivery systems, and for specific bacteria typing. In order to use phages in these applications, they are commonly purified by liquid chr...
An automatic tool is developed to identify microbiological data types using computer-vision and statistical modeling techniques. In bacteriophage (phage) typing, representative profiles of bacterial types are extracted. Currently, systems rely on the subjective reading of the profiles by a human expert. This process is time-consuming and prone to errors. The statistical methodology presented in...
PURPOSE To evaluate the abilities of these subtyping methods, we distinguished Salmonella Enteritidis (S. Enteritidis) isolated from food products and human clinical samples between 2009 and 2010 in Seoul using five subtyping methods. METHODS We determined the subtypes of 20 S. Enteritidis isolates from food and human sources using phage typing, antimicrobial susceptibility, pulsed-field gel ...
Introduction: Considering the changing geographical and temporal occurrence of Vibrio cholerae, there is a continuing need to monitor the strain characteristics and antibiotic resistance patterns of this pathogen. The present study was conducted to document the changing biology of V. cholerae isolates in and around Delhi, India, and the development of antibiotic resistance. Methodology: A total...
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