Lignin biodegradation is catalyzed in part by ligninases, also known as lignin peroxidases (1-4). We have cloned and sequenced the gene encoding ligninase isozyme H8 from the white-rot fungus Phanerochaete chrysosporium. The gene is interrupted by eight introns, ranging in size from 49 to 69 bp, which are distributed throughout the gene. Putative transcriptional control signals (underlined) inc...

Phenolic resins, phenol-formaldehyde polymers previously thought to be nonbiodegradable, are produced at an annual rate of 2.2 million metric tons in the United States for many industrial and commercial applications. Three independent lines of evidence established their biodegradability with the white-rot fungus Phanerochaete chrysosporium. Chromatic transformation of growth medium (yellow to p...

The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-...

The aim of the present work is to obtain a chemical-mechanical pulp (CMP) from poplar wood with improved properties, to be used in packing papers in place of more expensive softwood or hardwood pulp. For improving the CMP quality indicators, a preliminary treatment of the pulp has been carried out with a mixture of oxidizing enzymes produced from Phanerochaete chrysosporium, including lignin pe...

Mat of mycelia of Phanerochaete chrysosporium were immobilized on loofa-sponge discs, as an inert support, to obtain a suitable biosorbent .Mean amount of immobilized P.chrysosporium on loofa-sponge was 376 mgg of dry loofa-sponge. In this study immobilized P.chrysosporium and naked loofa-sponge discs were used as biosorbent to remove Pb (II) and Cd (II) from aqueous solution .At kinetic study ...

To improve the removal efficiency of heavy metals from wastewater, the surface of a fungal biomass was modified to obtain a high-capacity biosorbent for Cr(VI) in wastewater. The effects of pH, initial concentration, and sorption time on Cr(VI) removal by polyethylenimine (PEI)-modified Phanerochaete chrysosporium were investigated. The biomass adsorption capacity was significantly dependent on...