a gradient reversed-phase high performance liquid chromatography (hplc) method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (gnrh) antagonistic activity used in infertility treatment. the hplc method, which is used to determine cetrorelix in bulk and pharmaceutical dosage forms, was validated per ich guidelines. the chromatograph...

OBJECTIVES To develop and optimize quantitative HPLC method using 2,3-naphthalenedicarboxaldehyde (NDA) after simple and efficient solid phase extraction to determine the histamine in a biopharmaceutical (Histobulin™). METHODS The HPLC method was established using NDA-induced Histobulin and compared with the recently reported HPLC method using o-phthaldehyde (OPA). The validated NDA-applied H...

high performance liquid chromatography coupled with photodiode array detector (hplc-dad) has been reported to quantify isolated flavonoids or these compounds in complex biological matrices, such as chinese herbal drugs and products from factories. this work was designed, therefore, to develop an hplc-dad system to separate quercetin, luteolin and apigenin and to quantify them in extractive solu...

solid phase extraction (spe) cartridge embedded with octadecyl c18 chain bounded to silica particles were used for the isolation and trace enrichment of pesticides from water samples collected from cotton, rice growing and municipal areas. the eluents were analyzed with high performance liquid chromatography (hplc) using methanol (100%) as mobiles phase at different uv-visible wavelengths. ddt ...

Cyanobacteria produce a wide range of potent toxins, including hepatotoxic microcystins. HPLC methods for microcystin analysis and purification almost invariably include acetonitrile in the elution gradient mobile phase. The recent, acute, global acetonitrile shortage requires that adequate methods are available for microcystin analysis and purification without the need for acetonitrile. Here w...

A simple, rapid and validated HPLC method was developed for determination of Piracetam in film coated tablets. A Lichrosorb® (RP-18 column with a mobile phase consisting of acetonitrile – phosphate buffer (5:95v/v)) was used. Quantitative evaluation was performed at 205 nm. The HPLC method is selective, precise and accurate and can be used for routine analysis of preparations in pharmaceutical ...

the high mobility group (hmg) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (hplc). reversed-phase hplc under conditions of 50 mm triethylamine adjusted to ph 2.2 with phosphoric acid (solvent a) and 95% acetonitrile in water (solvent b) was used to separate proteins primarily on the basis of differences in the overall hydrophobicity....

The present study describes a simple, accurate, precise and cost effective UV-Vis Spectroscopic and RP-HPLC method for the estimation of Ibandronate sodium (IBN). The determination of Ibandronate sodium (IBN) was performed by both UV and RP-HPLC method using 215 nm as the determination wavelength. The drug was dissolved in NaOH solution (0.1N NaOH) for estimation in UV and in distilled water fo...

Objective: To develop and validate simple, rapid, precise, accurate, and economical UV spectrophotometric and reverse phase high performance liquid chromatographic methods for the estimation of alogliptin benzoate (AGP). Methods: UV spectrophotometric method was performed using UV/Vis double beam spectrophotometer with a spectral bandwidth of 1 nm and 1.0 cm matched quartz cells. The maximum ab...