Reactive oxygen species are believed to perform multiple roles during plant defense responses to microbial attack, acting in the initial defense and possibly as cellular signaling molecules. In animals, nitric oxide (NO) is an important redox-active signaling molecule. Here we show that infection of resistant, but not susceptible, tobacco with tobacco mosaic virus resulted in enhanced NO syntha...

Phenylalanine ammonia-lyase (PAL) is encoded by a small family of genes in Arabidopsis. We cloned and partially characterized one of these genes, PAL1. The deduced amino acid sequence is highly similar to PAL from bean, parsley, and rice. The promoter contains sequence elements homologous to two putative regulatory elements conserved among several phenylpropanoid genes. The regulation of the PA...

Phenylketonuria (PKU), a Mendelian autosomal recessive phenotype (OMIM 261600), is an inborn error of metabolism causing impaired postnatal cognitive development in the absence of treatment. We used the Pah(enu2/enu2) PKU mouse model to study oral enzyme substitution therapy with various chemically modified formulations of phenylalanine ammonia lyase (Av-p.C503S/p.C565S/p.F18A PAL). In vivo stu...

To better understand the molecular responses of plants to arbuscular mycorrhizal (AM) fungi, we analyzed the differential gene expression patterns of Lotus japonicus, a model legume, with the aid of a large-scale cDNA macroarray. Experiments were carried out considering the effects of contaminating microorganisms in the soil inoculants. When the colonization by AM fungi, i.e. Glomus mosseae and...

Two strains of callus have been isolated from bean hypocotyl and grown on a defined maintenance medium supplemented with 2 mg/l. 2:4-dichlorophenoxyacetic acid (2:4D) and 2% sucrose. Root initiation was observed in one strain and formation of nodules containing xylem and phloem in both strains after transfer to an induction medium supplemented with 1 mg/l. naphthyleneacetic acid, 0-2 mg/l. kine...

Tomato Lycopersicon esculentum Mill. is one of the most cultivated and widely consumed vegetables in world. However, it very susceptible to infection initiated by Fusariumoxysporum fruit rot, which shortens post-harvest life thus reduces market value. This disease can be regulated appropriately application synthetic fungicides. chemical fungicides constitute a serious health risk, have harmful ...

In Cameroon, tomato yields remain low due to attack by pathogens and insects. Phytophtora infestans (PI) is a phytopathogenic fungus responsible for the downy mildew of tomatoes, fungal disease enormous economic losses. To contribute search means control, stimulatory effect defence system extracts some plants was evaluated. Tomato were treated with aqueous macerates Callistemon citrinus (C. cit...

ABSTRACT The biological control, thinking about the integrated management, has been inserted with other management techniques to disease such as soybean anthracnose. aims of this work were verify action Trichoderma and Bacillus isolates in induction resistance mechanisms anthracnose a function seed treatment. statistical design was entirely randomised, 5 x 2 (agent species sampling times) facto...

Oleuropein, Hydroxytyrosol and Tyrosol are three major phenolic compounds present in olive with remarkable pharmacological effects. In this study, leaves and fruit of four olive cultivars including ‘Fishomi’, ‘Mary’, ‘Roghani’ and ‘Zard’ grown in the north of Iran were collected for comparative evaluation of the concentration of Oleuropein, Hydroxytyrosol and Tyrosol as well as antioxidant prop...

Phenylalanine ammonia-lyase (PAL) links the plant primary and secondary metabolisms, its product, trans-cinnamic acid, is derived into thousands of diverse phenylpropanoids. Bambusa oldhamii BoPAL4 has broad substrate specificity using L-phenylalanine, L-tyrosine, L-3,4-dihydroxy phenylalanine (L-DOPA) as substrates to yield p-coumaric caffeic respectively. The optimum reaction pH for three was...