THE methods employed for the estimation of pyruvic acid are largely based upon the reaction of its carbonyl group with phenylhydrazine. Its estimation in complex biological fluids where there are other compounds which react likewise is therefore difficult and, particularly when pyruvic acid exists in small quantities amidst these compounds, the estimation is unreliable even when controls are ru...

Iron is eliminated in the bile of normal dogs at a low but quite constant rate, 0.2 mg. per day. The feeding of large amounts of iron to normal dogs does not cause an increased iron excretion in the bile nor does the injection of considerable quantities of colloidal iron by vein. When red cell destruction is brought about by acetyl-phenylhydrazine the elimination of biliary iron may increase te...

A large number of foreign compounds, including many drugs, industrial pollutants, and environmental chemicals, can be oxidized under appropriate conditions to potentially toxic free radical intermediates. We evaluated the ability of the oxidants produced by the neutrophil myeloperoxidase system to generate free radical intermediates from several such compounds. Sodium hypochlorite or hypochloro...

Published in the Scandinavian Journal of Laboratory Animal Science an international journal of laboratory animal science Introduction In vertebrates, anemia is a common hematological disorder associated with several conditions such as drug toxicity, parasites, genetic or acquired defects, and blood loss (Criswell et al., 2000; Jollow & McMillan, 2001). The hemolytic activity of arylhydrazines, ...

Hepcidin, a key regulator of iron metabolism, decreases intestinal absorption of iron and its release from macrophages. Iron, anemia, hypoxia, and inflammation were reported to influence hepcidin expression. To investigate regulation of the expression of hepcidin and other iron-related genes, we manipulated erythropoietic activity in mice. Erythropoiesis was inhibited by irradiation or posttran...

O NE OF THE causes of an increase in mean corpuscular size is a pronounced reticulocytosis.’#{176} The increase in the volume of the red blood cells due to this cause is usually a temporary finding which follows a sudden loss of blood, a hemolytic crisis, or any reaction which acutely stimulates the hematopoietic system. During treatment of pernicious anemia with specific therapy, it is not unu...

The present study tested the hypothesis that zebrafish (Danio rerio) aquaporin-1a1 (AQP1a1) serves as a multi-functional channel for the transfer of the small gaseous molecules, CO2 and ammonia, as well as water, across biological membranes. Zebrafish embryos were microinjected with a translation-blocking morpholino oligonucleotide targeted to AQP1a1. Knockdown of AQP1a1 significantly reduced r...