Phosphoinositide lipid metabolism and prostacyclin production are implicated in endothelium dependent vascular relaxation in large blood vessels. To determine if these biochemical pathways might be involved in the regulation of microvascular tone in the retina, we measured the formation of 6-keto-prostaglandin-F1 alpha, the stable end product of prostacyclin, and inositol phosphates from 3H-lab...

Extracts from Escherichiu coli catalyze the formation of deoxycyticlme phosphates from cytidine 5’-phosphate (1). Optimal formation of deoxynucleotides required the addition of adenosine triphosphate and Mg* ions to the extract, and, after treatment with Dowex 2, a strong stimulation of the reaction by reduced triphosphopyridine nucleotide was observed. Similar results were obtained in extracts...

The reversible conversion between D-glucose 6-phosphate and D-fructose 6-phosphate catalyzed by yeast phosphoglucoisomerase was studied by phase sensitive two-dimensional 13C-[1H] EXSY NMR spectroscopy at 150.869 and 125.759 MHz, using 13C-enriched substrates in the C2 position of the D-hexose 6-phosphates. The shape of the build-up curves of the cross-peaks associated with the 13C2 resonances ...

In vitro "glycorandomization" is a chemoenzymatic approach for generating diverse libraries of glycosylated biomolecules based on natural product scaffolds. This technology makes use of engineered variants of specific enzymes affecting metabolite glycosylation, particularly nucleotidylyltransferases and glycosyltransferases. To expand the repertoire of UDP/dTDP sugars readily available for glyc...

The content of chlorides, nitrites, nitrates, phosphates and sulphates was used to classify 45 donkey's milk samples collected from different Italian regions. A method employing ion exchange chromatography with conductivity detector and chemical suppression was used. The quantitative results indicated phosphates (569.4-1304.4 mg kg(-1)) and chlorides (545.9-1757.9 mg kg(-1)) as being the most a...

  Two facile and low cost routes were used for the synthesis of IF-WS2 nanoparticles. In this paper, preparation of IF-WS2 nanoparticles with two different methods was investigated. In the first method, WO3 nanopa...

Antimycobacterially active salicylanilide diethyl phosphates were evaluated to identify their potential drug target(s) for the inhibition of several mycobacterial enzymes, including isocitrate lyase, L-alanine dehydrogenase (MtAlaDH), lysine ε-aminotransferase, chorismate mutase, and pantothenate synthetase. The enzymes are related to the nongrowing state of Mycobacterium tuberculosis. Salicyla...

This study examined the ability of the excitatory amino acid glutamate and its analogs to stimulate phosphatidylinositol metabolism in isolated cochlear nucleus tissue from young chicks. In the presence of lithium chloride, glutamate and (+/-)-1-aminocyclopentyl-trans-1,3-dicarboxylate (ACPD) stimulated the formation of inositol phosphates to levels significantly above unstimulated control leve...

Hollow magnetic Fe3O4@NH2-MIL-101(Fe) derived from metal-organic frameworks are fabricated through a general facile strategy. The synthetic parameters are regulated to control the shape of the as-prepared samples. The concentration of phosphates decreased sharply from the initial 0.60 to 0.045 mg.L(-1) with the exposure time in 50 minutes. The correlation between the most significant parameters...