Strain DS-1 is a small (0.8 μm in length and 0.2 μm in diameter) heterotrophic member of the α-Proteobacteria able to ω-oxygenate the commercial surfactant linear alkylbenzenesulfonate (LAS) and shorten the sidechain by β-oxidation to yield sulfophenylcarboxylates. The morphotype is widespread in cultures able to utilise LAS, and a second organism with similar characteristics, strain AN-8, is n...

Incubation of slices of the salt gland of the albatross with acetylcholine, which is the physiological secretogogue for this tissue, led to a 13-fold increase in the rate of incorporation of P(32) into phosphatidic acid and a 3-fold increase in the incorporation of P(32) and inositol-2-H(3) into phosphoinositide. The incorporation of P(32) into phosphatidyl choline and phosphatidyl ethanolamine...

In studying the control of thyroidal phosphatide metabolism it was found that osmotic shock could stimulate the z2P incorporation into lipids of surviving thyroid slices. The nature of this effect and its relation to thyrotropin stimulation of phosphatide turnover was examined by first incubating calf thyroid slices in hypotonic or isotonic medium for 15 min and then transferring the slices to ...

Previously we demonstrated high concentrations of desmosterol and docosahexaenoic acid (DHA, 22:6 n-3) in monkey testes and sperm. Desmosterol, a cholesterol precursor, is not present elsewhere in the body. High concentrations of DHA are found elsewhere only in the retina and brain. To examine the distribution of these compounds in the heads and tails of sperm, we separated them and determined ...

It is generally accepted that the behaviourally stimulant properties of D-amphetamine can be at­ tributed to its ability to release catecholamines from intraneuronal storage sites and block their re­ uptake lf 2. Since lipids constitute an essential part of cellular membranes 3, and there is some evidence that they may be involved in ion transport pro­ cesses in nerve membranes 4, it seems poss...

Lipogenesis in vitro from acetate-1-(14)C was studied in human preputial skin and abdominal skin. Radioactive lipids were separated by column chromatography on Florisil and by thin-layer chromatography on silica gel. Radioactivity was incorporated chiefly into the triglyceride, sterol, and polar lipid fractions, while lesser amounts of (14)C were found in the hydrocarbon, wax, diglyceride, mono...

Conventional liposomes normally shows frequent uptake by reticuloendothelial system(RES). To reduce the RES uptake and to prolong the biological half life researchers aimed to prepare sterically stabilized liposomes containing charged or neutral particles as surface modifier for targeting various organs for therapeutic or diagnostic purposes. Film method followed by ultracentrifugation and soni...

A mutant cell line (designated M.9.1.1) requiring ethanolamine for growth was derived from Chinese hamster ovary (CHO-K1) cells using 5-bromodeoxyuridine enrichment. The ethanolamine requirement was readily replaced by 20 MM phosphatidylserine and 10 FM lysophosphatidylethanolamine. When M.9.1.1 cells were supplemented with phosphatidyl['H]serine it was rapidly taken up, and subsequently decarb...

We report a methodology for the isolation of peroxisome membranes from the yeast Candida tropicalis pK233 grown on oleic acid, and the characterization of the polypeptide and lipid compositions of these membranes. Peroxisomes purified in either sucrose or Nycodenz gradients are treated with Tris-HCl (pH 8.5) and then with sodium carbonate (pH 11.5) to yield a final peroxisome membrane preparati...