Phosphodiesterase 4B (PDE4B) is a member of the phosphodiesterase family of proteins that plays a critical role in regulating intracellular levels of cyclic adenosine monophosphate (cAMP) by controlling its rate of degradation. It has been demonstrated that this isoform is involved in the orchestra of events which includes inflammation, schizophrenia, cancers, chronic obstructive pulmonary dise...

A cAMP-specific phosphodiesterase was found that is stimulated by binding to the regulatory subunit of cAMP-dependent protein kinase, PKA-R, from either Dictyostelium or mammals. The phosphodiesterase is encoded by the regA gene of Dictyostelium, which was recovered in a mutant screen for strains that sporulate in the absence of signals from prestalk cells. The sequence of RegA predicts that it...

Here we show that administration of the phosphodiesterase type 4 (PDE4) inhibitor rolipram into the basolateral complex of the amygdala (BLA) at a specific time interval after training enhances memory consolidation and induces memory persistence for novel object recognition (NOR) in rats. Intra-BLA infusion of rolipram immediately, 1.5 h, or 6 h after training had no effect on retention tested ...

Correspondence: *Inserm U408, Unité de Pneumologie de l'Hôpital Bichat, Paris, France. ** and Dept of Thoracic Medicine, National Heart and Lung Institute, London, UK. This issue of the European Respiratory Journal starts a series of short reviews based on a Satellite Meeting held during the European Respiratory Society Annual Meeting in Nice in October 1994. The symposium was supported by Byk ...

Two types of methods have been used for the elucidation of specificity (or preference) of nucleases when complex polynucleotides are used as substrates. One method depends on the identification of products in the digest. I f a particular combination (or combinations) is missing, it means that this combination is preferentially attacked. Thus, in the case of pancreatic ribonuclease, sequences Py...

As a part of a program of studies aiming at the development of methods for the end group and sequential analysis of polynucleotides (l), we have been investigating the properties and mode of action of certain phosphodiesterases. Studies of this kind have been facilitated by the availability of a variety of substrates and, in particular, synthetic oligonucleotides of known size and structure (2)...

BACKGROUND Inhaled nitric oxide produces potent pulmonary vasodilation by activating soluble guanylate cyclase and increasing smooth muscle cell concentrations of cyclic guanosine monophosphate. However, responses are often nonsustained, and clinically significant increases in pulmonary vascular resistance have been noted on its acute withdrawal. In vitro and in vivo data suggest that inhaled n...

The immunological method was therefore shown to be capable of isolating and partially purifying membranes from a whole rat liver with approx. 40% yield within 50min from breaking cells and with only minimal exposure to temperatures below physiological ones. Electron microscopy of the membrane preparation showed organelles, including mitochondria, sometimes enclosed inside larger membranous elem...