After synthesis during the early log phase, the concentrations of ubiquinone and cytochromes did not vary during the growth cycle of Azotobacter vinelandii, when grown with either high or low aeration on nitrogen-free or urea-containing media. The level of aeration had no effect on the concentrations of the electron carriers synthesized, but affected the growth rate. On urea-containing medium, ...

The presence and activities of urease genes were investigated in 49 clinical, food, and environmental Bacillus cereus isolates. Ten strains were shown to have urease genes, with eight of these strains showing growth on urea as the sole nitrogen source. Two of the urease-positive strains, including the sequenced strain ATCC 10987, could not use urea for growth, despite their capacities to produc...

Nitrogen is an important element in the life of a plant. A wide range of nitrogen-containing compounds is available in the world market, with different formulations and efficiencies. Urea is present in most fertilizer applications; however, nano-nitrogen products are becoming popular although there is only limited information on their field efficiency. Thus, this paper studied the foliar applic...

Currently, the mechanism(s) responsible for the regulation of urea transporter B (UT-B) expression levels in the epithelium of the rumen remain unclear. We hypothesized that rumen fermentation products affect ruminal UT-B expression. Therefore, the effects of short-chain fatty acids (SCFA), pH, ammonia, and urea on mRNA and protein levels of UT-B were assayed in primary rumen epithelial cell cu...

Facilitative UT-B urea transporters enable the passage of urea across cell membranes. Gastrointestinal urea transporters are thought to play a significant role in the urea nitrogen salvaging process that occurs between mammalian hosts and their gut bacteria. This study investigated the expression of UT-B urea transporters in different segments of human colon. Immunoblot analysis showed that hum...

DCA was administered in two separate experiments to rats in amounts sufficient to cause marked elevation of the blood pressure. It was then abruptly withdrawn. Following this treatment, observations of blood pressure, blood urea nitrogen, plasma potassium, and plasma volume were carried out for over a year. Approximately one-third of the animals so treated developed a sustained, permanent hyper...

Objective Many livestock owners use the granular form of urea nitrogen during late summer and fall trying to increase forage growth for “stockpiled” forage. Livestock are then allowed to graze the “stockpile” at a later date when other forages no longer are growing or available. This practice extends the grazing season and reduces the need for higher priced stored feed. Urea is a common nitroge...

Urea, a non-protein nitrogen for dairy cows, is rapidly hydrolyzed to ammonia by urease produced by ureolytic bacteria in the rumen, and the ammonia is used as nitrogen for rumen bacterial growth. However, there is limited knowledge with regard to the ureolytic bacteria community in the rumen. To explore the ruminal ureolytic bacterial community, urea, or acetohydroxamic acid (AHA, an inhibitor...

The model rumen Firmicutes organism Ruminococcus albus 8 was grown using ammonia, urea, or peptides as the sole nitrogen source; growth was not observed with amino acids as the sole nitrogen source. Growth of R. albus 8 on ammonia and urea showed the same growth rate (0.08 h(-1)) and similar maximum cell densities (for ammonia, the optical density at 600 nm [OD600] was 1.01; and for urea, the O...

the aim followed in the present study was to investigate the effects of different metabolizable protein (mp) levels on holstein cows, milk yield and composition, and as well on concentration of plasma metabolites in the cow,s early lactation. twenty - four holstein cows(bw 580±23, dim 30±7, parity 2.4±0.5) were randomly assigned to each one of the 4 dietary treatments containing 1) 10.60% (diet...