BACKGROUND Lactic acid bacteria (LAB) play an important role in agricultural as well as industrial biotechnology. Development of improved LAB strains using e.g. library approaches is often limited by low transformation efficiencies wherefore one reason could be differences in the DNA methylation patterns between the Escherichia coli intermediate host for plasmid amplification and the final LAB ...

The incidence of pancreatic carcinoma, a gastrointestinal malignancy, is on the increase and effective therapeutic strategies are therefore required. This study aimed to construct a recombinant plasmid pcDNA3.1(-) shCEACAM6-yCDglyTK from CEACAM6 targeting shRNA and the fusion suicide gene yCDglyTK for inhibition of SW1990 human pancreatic carcinoma cell growth and invasion. A plasmid containing...

A major inconvenience in cloning a dominant mutation in Saccharomyces cerevisiae is the requirement for the construction of a genomic library from the mutant strain (1). To alleviate the need for library construction, we present an alternative that is based on the ability of yeast cells to carry out precise and efficient homologous recombination (2). In principle, a dominant mutation may be clo...

Yeast episomal shuttle vectors (YEp type) are commonly used in fundamental research and biotechnology whenever elevated product levels are desired. Their instability, however, poses an impediment not only in industrial scale fermentation. In order to analyse instability which might be linked to plasmid structure, a series of YEp type plasmids that are identical in size has been assembled, diffe...

1 Animal Scientist, D.Sc., Adjunct Professor, Department of Animal Science, Universidade Federal de Viçosa, ViçosaMG, 36571-000, CNPq and INCT-Ciência Animal researcher ([email protected]) 2 Animal Scientist, D.Sc., Titular Professor, Department of Animal Science, Universidade Federal de Viçosa, Viçosa-MG, Brazil, 36571-000, CNPq and INCT-Ciência Animal researcher ([email protected]) 3 Agronomy Engi...

This study was carried out to evaluate the plasmid mediated drugs resistance among wound bacteria isolates obtained from (352 swabs specimens) patients attending some hospitals in Kano State (National Orthopaedic Hospital Dala, Aminu Teaching Hospital, Infectious Diseases Bichi General and Wudil Hospital). Swab specimens were aseptically using randomized sampling method cultured on Blood MacCon...

background: recently, the use of t7 rna polymerase instead of other viral and cellular promoters is increasing due to high efficacy of transcription in the cell cytoplasm by this polymerase. in order to translate the transcripts produced by t7 rna polymerase in mammalian cell lines, it is necessary to include internal ribosome entry site (ires) sequences. in addition, if sequence of poly a sign...

the present study aimed to evaluate the antibiotic resistance pattern of escherichia coli strains isolatedfrom animals to ascertain the levels of antibiotic resistance pervasiveness. a total of 28 e. coli strains wereisolated from faecal samples and the antibiotic resistance pattern of e. coli strains was determined by meansof disc diffusion assay. the resistance pattern determined for all stra...