Comparative genomics of several strains of Erwinia amylovora, a plant pathogenic bacterium causal agent of fire blight disease, revealed that its diversity is primarily attributable to the flexible genome comprised of plasmids. We recently identified and sequenced in full a novel 65.8 kb plasmid, called pEI70. Annotation revealed a lack of known virulence-related genes, but found evidence for a...

-Actin mRNA is often used for normalization in gene expression experiments. We describe a sensitive, rapid and specific quantitative assay for the cytoplasmic ovine -actin mRNA. The assay was based on the polymerase chain reaction (PCR) with real-time fluorescence resonance energy transfer (FRET) measurements to amplify cDNA products reverse transcribed from mRNA. A part of the ovine -actin seq...

Abstract One of the most severe side effects systemic lupus erythematosus (SLE) is nephritis (LN). To search for potential therapeutic targets in SLE crucial progression SLE. In this study, we selected C57BL/6J mice as controls and MRL/lpr an LN model obtained dual specificity phosphatase 2 (DUSP2)-overexpressed by injecting AAV-DUSP2 plasmid into tail vein. Then, proteinuria, urea nitrogen, ds...

OBJECTIVES The nvCT has a 377 bp plasmid deletion that has caused commonly used nucleic acid amplification tests to generate false negative results. In Swedish counties nvCT has been reported to account for up to 65% of all chlamydia cases in 2007. The aim was to evaluate the CTQ DNA amplification assay for detection of the new Chlamydia trachomatis variant (nvCT) in urine specimens from a labo...

objective(s):n-myc downstream regulated gene 2 (ndrg2) is a candidate gene for tumor suppression. the expression of ndrg2 is down-regulated in several tumors including lung cancer. the aim of this study was to explore the effect of ndrg2 overexpression on invasion, migration, and enzymatic activity of matrix metalloproteinase-2 (mmp-2) and -9 (mmp-9) in human lung adenocarcinoma a549 cells.  ma...

Polyethylenimine (PEI) is one of the most potent non-viral vectors. We have developed a lactosylated PEI (Lac-PEI) to enhance cell-specific transfection and have shown that Lac-PEI is more efficient than unsubstituted PEI for gene transfer into immortalized cystic fibrosis airway epithelial SigmaCFTE29o-cells. As both intact PEI/plasmid and Lac-PEI/plasmid complexes are found in the cell nucleu...

Background. Various cationic polymers have been proposed for use as carriers of genetic materials in biotechnology and biomedicine. The poly(2-dimethylamino)ethyl-methacrylate (poly(DMAEMA)) demonstrated high transfection efficiency low cytotoxicity when used a gene delivery system. aim the present study was to evaluate ability poly(DMAEMA)-block-poly(N-vinylpyrrolidone)-co-(butyl-acrylate)-co-...

The excess linking deficit of plasmid DNA from topoisomerase I-defective bacteria (topA mutants) results mainly from transcription and is commonly ascribed to unbalanced relaxation of transcription-induced twin-supercoiled domains. This defect is aggravated in genes for membrane-binding proteins (such as the tet gene) where anchoring of the transcription complex to the bacterial membrane is tho...

BACKGROUND Plasmodium ovale is comprised of two genetically distinct subspecies, P. ovale curtisi and P. ovale wallikeri. Although P. ovale subspecies are similar based on morphology and geographical distribution, allelic differences indicate that P. ovale curtisi and P. ovale wallikeri are genetically divergent. Additionally, potential clinical and latency duration differences between P. ovale...