Reactive oxygen species can be generated by daily exposure of the skin to ultraviolet light and may cause some subchronic and chronic skin disorders. The aim of this study was to investigate a possible preventive role of alpha-tocopherol acetate (ATA) on ultraviolet B (UVB) induced peroxidation by assessing lipid peroxide (LPO) levels and activity of reactive oxygen scavenging enzymes including...

richlorotrifluoroethane (CFC-113) T has been widely used to remove machining oils from metals after those metals have been stamped and formed. In many cases, removal of the hydrocarbon machining oils is necessary before the metals can undergo further processing such as electroplating. In other situations, the stamping occurs as the last step in the process and the formed metal parts must be cle...

High-throughput genomic approaches to gene function or target identification have led to the development and implementation of the 96-well format for many standard molecular biology manipulations. The apparatus described here, a Multichannel Plating Unit, is designed to plate out individual cultures efficientlyfrom standard 96-well culture blocks. Following transformation, aliquots of culture a...

objective: ionizing radiation interacts with biological systems to induce excessive fluxes of free radicals that attack various cellular components. melatonin has been shown to be a direct free radical scavenger and indirect antioxidant via its stimulatory actions on the antioxidant system.the aim of this study was to evaluate the antioxidant role of melatonin against radiation-induced oxidativ...

We have previously reported that, when 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer was used in the growth medium to control pH fluctuations during the 21-day expression period of our human cell hybrid (HeLa x skin fibroblast) transformation assay, the yield of radiation-induced neoplastically transformed foci after 7 Gy of gamma-irradiation was suppressed. We now demonstrate that ...

Raw breast fillets were divided into two groups and either vacuum or aerobically packaged. The fillets in each group were subdivided equally into two groups and then irradiated at 0 or 3 kGy using a Linear Accelerator. After 0, 3 and 7 days of storage at 4 °C, fillets were cooked in an 85 °C water bath (cook-in-bag) to an internal temperature of 74 °C. Oxidation-reduction potential (ORP) of raw...

The apparatus used to supply the cathode radiation was a high voltage electrostatic generator of the Van de Graaff type (Trump and Van de Graaff, 1948; Proctor and Goldblith, 1951), which was operated at a constant potential of about 2.8 megavolts and with a beam current of 2.5 microamperes. A dose of 10,000 rep was delivered in about 1 sec. The salts used were of the reagent or cp grade. The o...

A process of emulsifying and centrifuging abattoir specimens before plating out is described. Brucella abortus was isolated more successfully by this process than by conventional methods, especially in low grade infections. The 5 different media used were equally effective in our attempts at isolation, but growth did not necessarily occur on all 5 plates. In dairy cows, specimens from supra-mam...

Kelner (1949a) first reported that mutations to phage resistance induced in Escherichia coli by ultraviolet light could be reversed by immediate irradiation with white light. In addition he noted that end-point mutations in contrast to zero-point mutations were not reversed by visible light (1949b). At the same time Novick and Szilard (1949) reported that light-reactivation of E. coli reduced t...