With one Plate SUMMARY Methods for the demonstration of the various cytological elements of bacteria are described. The specialized techniques applicable to pleuropneumonia-like organisms and L-forms of bacteria are also presented. D URING the last decade new methods for the study of various morphological features of bacteria and other minute organisms have been developed including techniques f...

Nopadon Pirarat* Yumi Une** Yasuo Nomura** IMMUNOHISTOCHEMICAL DETECTION OF FELINE VIRAL RHINOTRACHEITIS IN A CAT A female, 10-month old, mixed breed cat showed clinical signs of severe dehydration, purulent nasal discharge, dyspnea, severe crepitating lung sounds and no response to antibiotic treatment. Upon necropsy, histopathology revealed severe, diffuse, fibrinopurulent pleuropneumonia and...

A selected colony free from ocular infection with pleuropneumonia-like organisms of the conjunctival type was developed from young Princeton mice delivered by cesarean section and reared by uninfected Swiss foster mothers. Young mice from this colony continued to show sporadic ocular manifestations similar to those observed in infected mice. This reaction was non-inflammatory in nature. The org...

Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (C...