Primary myelofibrosis (PMF) is a clonal hematologic malignancy characterized by BM fibrosis, extramedullary hematopoiesis, circulating CD34+ cells, splenomegaly, and a propensity to evolve to acute myeloid leukemia. Moreover, the spleen and BM of patients harbor atypical, clustered megakaryocytes, which contribute to the disease by secreting profibrotic cytokines. Here, we have revealed that me...

We investigate equilibrium observables for molecules near metals by employing a potential of mean force (PMF) that takes level broadening into account. Through comparison with exact data, we demonstrate that this PMF approach performs quite well, even for cases where molecule-electrode couplings depend on nuclear position. As an application, we reexamine the possibility of hysteresis effects wi...

Using the Jagla model potential we calculate the potential of mean force (PMF) between hard sphere solutes immersed in a liquid displaying water-like properties. Consistent estimates of the PMF are obtained by (a) umbrella sampling, (b) calculating the work done by the mean force acting on the hard spheres as a function of their separation, and (c) determining the position dependent chemical po...

(median survival, 109 months) and high-risk (median survival, 26 months). External validity and discriminating power are the main attributes of any prognostic classification. Unfortunately, both remain unsolved,3,4 probably because statistical validity does not always mean clinical value. For this reason, we assembled an unprecedented large number of PMF patients and worked only with variables ...

The molecular pathogenesis of primary mielofibrosis (PMF) is still largely unknown. Recently, single-nucleotide polymorphism arrays (SNP-A) allowed for genome-wide profiling of copy-number alterations and acquired uniparental disomy (aUPD) at high-resolution. In this study we analyzed 20 PMF patients using the Genome-Wide Human SNP Array 6.0 in order to identify novel recurrent genomic abnormal...

We present a statistical mechanical description of biomolecular hydration that accurately describes the hydrophobic and hydrophilic hydration of a model alpha-helical peptide. The local density of water molecules around a biomolecule is obtained by means of a potential-of-mean-force (PMF) expansion in terms of pair- and triplet-correlation functions of bulk water and dilute solutions of nonpola...

Background: The discovery of somatic acquired mutations of JAK2 (V617F) in Philadelphia-negative myeloproliferative neoplasms (Ph-negative MPNs) including polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF) has not only improved rational disease classification and prognostication but also brings new understanding insight into the pathogenesis of diseases. Dos...

In the present work, we investigated polycyclic aromatic hydrocarbons (PAHs), metals and ions of indoor outdoor PM2.5 from 80 samples collected in microenvironment a kindergarten located Belgrade city center during weekdays, March to May 2010. The daily concentrations were much higher than WHO guidance value. Results show similar factor profiles identified by principal component analysis (PCA) ...

The objective of this research was to evaluate the cause of fertility reduction after chemotherapy.Cyclophosphamide is a common chemotherapy and immunosuppressive agent used for the treatment of awide range of neoplastic and some auto-immune diseases. In the present study, morphometrical changes inthe ovary and uterus of 12 5–6-week-old female Syrian mice after intraperitoneal injection of 75 m...

Ambient non-refractory PM1 aerosol particles were measured with an Aerodyne High Resolution Timeof-Flight Aerosol Mass Spectrometer (HR-AMS) at an elementary school 18 m from the US 95 freeway soundwall in Las Vegas, Nevada, during January 2008. Additional collocated continuous measurements of black carbon (BC), carbon monoxide (CO), nitrogen oxides (NOx), and meteorological data were collected...