We review the main results obtained in the theory of schemata in genetic programming (GP), emphasizing their strengths and weaknesses. Then we propose a new, simpler definition of the concept of schema for GP, which is closer to the original concept of schema in genetic algorithms (GAs). Along with a new form of crossover, one-point crossover, and point mutation, this concept of schema has been...

In this paper we first review the main results obtained in the theory of schemata in Genetic Programming (GP) emphasising their strengths and weaknesses. Then we propose a new, simpler definition of the concept of schema for GP which is quite close to the original concept of schema in genetic algorithms (GAs). Along with a new form of crossover, one-point crossover, and point mutation this conc...

In the neurodegenerative disease multiple system atrophy (MSA), α-synuclein misfolds into a self-templating conformation to become a prion. To compare the biological activity of α-synuclein prions in MSA and Parkinson's disease (PD), we developed nine α-synuclein-YFP cell lines expressing point mutations responsible for inherited PD. MSA prions robustly infected wild-type, A30P, and A53T α-synu...

A method has been developed for the rapid isolation of human phosphoglucose isomerase by substrate-induced elution from phosphocellulose. The high degree of selectivity of the elution provides homogeneous, crystalline enzyme from erythrocytes with a 30,000-fold purification and an overall yield of approximately 70 %. A genetic variant form of the enzyme (Singh variant) has also been isolated fr...

As the affordability, performance and availability of Next Generation Sequencing (NGS) increases, sequencing data accumulates and with it the need to analyse it for useful information about the biological systems under study. Among the disciplines looking to gain insights from sequencing data is cancer research, which seeks to answer questions on cancer development, progression and treatment op...

Growth and Differentiation Factor 5 (GDF5) is a secreted growth factor that belongs to the Bone Morphogenetic Protein (BMP) family and plays a pivotal role during limb development. GDF5 is a susceptibility gene for osteoarthritis (OA) and mutations in GDF5 are associated with a wide variety of skeletal malformations ranging from complex syndromes such as acromesomelic chondrodysplasias to isola...

Quantum theory of hydrogen atoms distribution between two complementary nucleotide bases in DNA double helix at moment of replication has been proposed in this work. It bases on two mechanisms of proton tunneling: the Andreev-Meyerovich mechanism with spontaneous phonon radiation and the Kagan-Maximov (Flynn-Stoneham) mechanism at phonon scattering. According to the presented model, the probabi...

Living systems are subject to constant evolution. The environment can be considered as a nutriment shared by all the population. This alllows certain individuals, charaterized by a ’trait’ to better develop but also to change the environment. This leads to select the ’best adapted trait’ in the population. On the other hand the population can develop with small variance on the trait (mutations)...

An electrochemiluminescence-polymerase chain reaction (ECL-PCR) method for point mutation detection has been developed. The target is amplified using a tris (bipyridine) ruthenium (TBR)-labeled forward and a biotinylated reverse primer. The amplification products are digested with specific restriction enzyme, then captured onto streptavidin-coated paramagnetic beads, and detected by measuring t...

Desulfurization protein named DszC from Rhodococcus erythropolis is the key enzyme for biodesulforization of dibenzothiophene (DBT) in 4S pathway, which is a pathway with four enzymes. DszC enzyme biodesulfurizes DBT and its derivatives in oil components and biphasic systems. It functions well at the oil- water interface. In this study point mutation performed in DszC enzyme regarding to increa...