Objectives As efforts to control COVID-19 continue, we simulate hypothetical emergence of wild poliovirus assuming an immunologically naïve population. This differs from the current global experience with polio and serves as a model for responding future pandemics.Methods Applying established model, assume fully susceptible population polioviruses, independently introduce virus properties each ...

The emergence of vaccine-derived polioviruses (VDPVs) in patients with Primary Immunodeficiency (PID) is a threat to the polio-eradication program. In first its kind pilot study for successful screening and identification VDPV excretion among PID India, enteroviruses were assessed stool specimens 154 across India period two years. A total 21.42% tested positive enteroviruses, 2.59% (PV), wherea...

vaccines to prevent poliomyelitis have been available for 48 years, paralytic disease continues to occur in some areas, as reported by Kohler et al. 1 elsewhere in this issue of the International Journal of Epidemiology. Let us hope that by the 50th anniversary of the development of the first successful polio-myelitis vaccine all transmission of wild-type polioviruses will have ceased. The Worl...

Oral poliovirus vaccine can mutate to regain neurovirulence. To date, evaluation of these mutations has been performed primarily on culture-enriched isolates by using conventional Sanger sequencing. We therefore developed a culture-independent, deep-sequencing method targeting the 5' untranslated region (UTR) and P1 genomic region to characterize vaccine-related poliovirus variants. Error analy...

Studies on the fate of type 1 polioviruses in two common species of flies were carried out. The amount of virus in carcasses and excreta at different times was determined by the plaque assay method. Flies and their excreta remained infective for 11 days when kept at room temperature or when incubated at 36 degrees C. for 2 hours a day. Flies remained infective for 3 months when kept in hibernat...

The 2A proteins of the Picornaviridae enterovirus genus are small cysteine proteinases that catalyze essential cleavages in the viral polyprotein in cis and in several cellular proteins in trans. In addition, 2A has been implicated in the process of viral RNA replication, independent of its protease functions. We have generated viable polioviruses that encode 2A proteins containing fluorescent ...

In 1988, the World Health Assembly resolved to eradicate poliomyelitis (polio). Since then, wild poliovirus (WPV) cases have declined by >99.9%, from an estimated 350,000 cases of polio each year to 74 cases in two countries in 2015 (1). This decrease was achieved primarily through the use of trivalent oral poliovirus vaccine (tOPV), which contains types 1, 2, and 3 live, attenuated polioviruse...