Abstract. Introduction. Polyhydroxyalkanoate (PHA) is a useful, carbon-neutral polymer that can be produced from renewable carbon sources by microorganisms, making it sustainable and environmentally friendly material. Currently, production of the biopolymer not competitive in terms cost yield; however, different bacterial strains may provide economic viability, especially since substituting bio...

Despite being used as a common platform for the commercial production of many biochemicals, Bacilli are often overlooked source industrial polyhydroxyalkanoates (PHAs), biodegradable plastic replacements. In addition to having robust expression system, lack lipopolysaccharides and ease lysis make an attractive host PHAs. this work, Bacillus megaterium strain was engineered generate poly(3-hydro...

Polyhydroxyalkanoates (PHAs) are natural polyesters of increasing biotechnological importance that are synthesized by many prokaryotic organisms as carbon and energy storage compounds in limiting growth conditions. PHAs accumulate intracellularly in form of inclusion bodies that are covered with a proteinaceous surface layer (granule-associated proteins or GAPs) conforming a network-like surfac...