In this work we study the optimization of a polyhydroxyalkanoates (PHA) production process by mixed cultures based on a detailed hybrid metabolic model. The metabolic network under consideration was first decomposed into its fundamental pathways using the elementary flux modes (EFM) technique. Then, a dynamical hybrid semi-parametric model was formulated, which allowed to identify the EFM kinet...

Production of polyhydroxyalkanoates (PHAs) by mixed microbial cultures utilising the organic content of wastewaters is one of the technologies studied in the EU project ROUTES. When comparing the life-cycle environmental impacts of simultaneous wastewater treatment and production of PHA-rich biomass to traditional wastewater and solids treatment, the handling of this multi-functionality is crit...

This work describes the production of polyhydroxyalkanoates (PHA) as a side stream process on a municipal waste water treatment plant (WWTP) and a subsequent analysis of the production potential in Germany and the European Union (EU). Therefore, tests with different types of sludge from a WWTP were investigated regarding their volatile fatty acids (VFA) production-potential. Afterwards, primary...

The activated sludge process is commonly used to treat wastewater by aerobic oxidation of organic pollutants into carbon dioxide and water. However, several nonoxidative mechanisms can also contribute to removal of organics. Sorption onto activated sludge can remove a large fraction of the colloidal and particulate wastewater organics. Intracellular storage of, e.g., polyhydroxyalkanoates (PHA)...

This work examines the potential of the predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator of other Gram-negative bacteria, as an external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures. The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) produced naturally by Pseudomonas putida and Cupriavidus necator,...

The production of bacterial polyesters, polyhydroxyalkanoates (PHAs), has been improved by several rational approaches such as overexpression and/or engineering of the enzymes directly related to PHA biosynthetic pathways. In this study, a new approach at transcription level has been applied to a new category of the copolymer of lactate (LA) and 3-hydroxybutyrate (3HB), P(LA-co-3HB). When the 4...

Received 16.11.2015, received in revised form 17.01.2016, accepted 02.03.2016 The paper describes research of biomedical applications of experimental polymer devices prepared from polyhydroxyalkanoates (PHAs) in the Institute of Biophysics SB RAS and Siberian Federal University (Krasnoyarsk, Russia) between 2000 and 2012. The high-purity PHA specimens were investigated and then used to prepare ...