Perivascular lesions within the central nervous system (CNS) of rats with hyperacute experimental autoimmune encephalomyelitis (HEAE) contained large numbers of peripheral blood mononuclear cells (PBMC) and polymorphonuclear leukocytes (PMN), cells enzymatically capable of producing reactive nitrogen and oxygen intermediates (RNI and ROI), which, in excess, are mediators of tissue damage. PBMC ...

Candida albicans is a polymorphic opportunistic fungus that can cause life-threatening systemic infections following hematogenous dissemination in patients susceptible to nosocomial infection. Neutrophils form part of the innate immune response, which is the first line of defense against microbes and is particularly important in C. albicans infections. To compare the transcriptional response of...

We evaluated the sensitivity of a DNA amplification test for the detection of Mycobacterium avium in blood samples using different blood components and different DNA extraction methods. M. avium-inoculated blood samples were processed to obtain separate blood components: peripheral blood mononuclear cells (PBMCs), polymorphonuclear cells (PMNCs), and whole-blood sodium dodecyl sulfate (SDS)-lys...

Four Macaca nemestrina monkeys were inoculated in the conjunctiva with Chlamydia trachomatis (strain E) at 6 weeks of age. A fifth monkey was inoculated with HeLa cell materials only. Ten weeks later, all monkeys were reinoculated with either strain E or strain C. All inoculated monkeys were susceptible to infection with C. trachomatis as documented by fluorescent antibody staining of smears an...

background: there is evidence that mesangial cell structural changes contribute to the pathogenesis of diabetic nephropathy. to gain better insight into the mechanisms responsible for this issue, present study focused on effect of cord blood mononuclear cells (mncs) derived cd133 positive cells on mesangial cell structure and function. materials and methods: the animals were randomly divided in...

Background: NK (natural killer) and NKT (natural killer T) cells, as components of innate immune system, play a crucial role in tumor progression and dissemination. Objective: To investigate the percentages of NK cells, NKT cells, iNKT (invariant natural killer T) cells, total T lymphocytes as well as activated T lymphocytes, in tumor draining lymph nodes (TDLN...

Introduction: By relying on polymorphonuclear (PMN) cells, stromal vascular fraction (SVF) and platelet-rich plasma (PRP) contribute significantly in the healing process. Through anal trauma model, this study examined effect of SVF PRP combination PMN counts vivo. Method: Three groups twenty-eight eligible Wistar rats model randomly assigned to receive (Group A), normal saline B), or healthy co...

This study identifies and compares the distribution of mononuclear cells in the mammary gland tissues and milk of healthy and chronically infected with Staphylococcus aureus cows. Somatic cell counts (SCCs) during the 3 months before the study were > 1 x 10(6) cell/ml in the infected quarters and < 1 x 10(5) cell/ml in the infection-free quarters. Immediately after slaughter, samples from the t...

1. Immunity to reinfection is a function of the increased capacity of the mononuclear phagocytes to destroy tubercle bacilli and varies directly with the extent of the primary lesion; however it is rarely sufficient to annihilate completely the microorganism. This acquired immunity is superimposed on the natural resistance of a given organ. 2. In the presence of sufficient immunity, such as occ...