BACKGROUND Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function. METHODOLOGY/PRINCIPAL FINDINGS We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. We demonstrated that Populus protoplasts ...

poplar trees are appropriate species for plantation and forest rehabilitation because of fast growing and high wood yield, as well as adaptability to unfavorable climate. salinization of soil and high temperature are the inhibitory factors in khuzestan province. populus euphratica is a tree species that tolerates soil salinity and high temperature. acclimatization of cut seedling of three popul...

Halomorphic soils cover a significant area in the Vojvodina region and represent ecological economic challenges for agricultural forestry sectors. In this study, four economically important Serbian poplar clones were compared according to their biochemical transcriptomic responses towards mild severe salt stress select most tolerant afforestation of halomorphic soils. Three prospective Populus ...

We report the draft genome of the black cottonwood tree, Populus trichocarpa. Integration of shotgun sequence assembly with genetic mapping enabled chromosome-scale reconstruction of the genome. More than 45,000 putative protein-coding genes were identified. Analysis of the assembled genome revealed a whole-genome duplication event; about 8000 pairs of duplicated genes from that event survived ...

Leaf expansion in the fast-growing tree, Populus x euramericana was stimulated by elevated [CO(2)] in a closed-canopy forest plantation, exposed using a free air CO(2) enrichment technique enabling long-term experimentation in field conditions. The effects of elevated [CO(2)] over time were characterized and related to the leaf plastochron index (LPI), and showed that leaf expansion was stimula...

This work is the first attempt at using Granier sap sensors on Populus nigra, Populus x euramericana cv I45/51 and Salix alba for the monitoring of sap flows in an active floodplain over two consecutive years. The main characteristic of these diffuse porous trees is their capacity to use several tree rings for xylem sap transfer. Results showed that the sap flux densities remained homogeneous o...

Isochorismate synthase (ICS) converts chorismate to isochorismate for the biosynthesis of phylloquinone, an essential cofactor for photosynthetic electron transport. ICS is also required for salicylic acid (SA) synthesis during Arabidopsis defense. In several other species, including Populus, SA is derived primarily from the phenylpropanoid pathway. We therefore sought to investigate ICS regula...