CsA, bovine serum albumin, and β-nicotinamide adenine dinucleotide 2’-phosphate reduced tetrasodium salt hydrate (NADPH), were purchased from Sigma-Aldrich (St. Louis, MO). Cyclosporine D (internal standard for clinical study) and cyclosporine B (internal standard for in vitro study) were purchased from LKT Laboratories (St. Paul, MN). The CsA metabolites used for calibration curves and quality...

Hypothermic arrest, potassium arrest, and ischemic arrest, either singly or in combination, with or without coronary perfusion were studied in an isolated perfused rat heart preparation. Procedures that permitted the maintenance of high cellular levels of adenosine triphosphate (ATP) and creatine phosphate during arrest, e.g., coronary perfusion with hypothermic solutions or solutions containin...

Certain bivalent cations, particularly Mg(++) and Mn(++), can be absorbed by yeast cells, provided that glucose is available, and that phosphate is also absorbed. The cation absorption is stimulated by potassium in low concentrations, but inhibited by higher concentrations. From the time course studies, it is apparent that the absorption rather than the presence of phosphate and the potassium i...

The dye reagent is buffered by M/10 phosphate salts at pH 7.4 and is prepared by dissolving 12.07 gm. anhydrous disodium hydrogen phosphate powder, 2.04 gm. potassium dihydrogen phosphate crystals, 2.60 gm. sodium arsenite powder, and 2.10 gm. sodium fluoride powder in 1 L. of distilled water. Sufficient 2,6-dichlorobenzenoneindophenol sodium is added to bring the absorbance of the solution to ...

Geoactive soil fungi were investigated for phosphatase-mediated uranium precipitation during growth on an organic phosphorus source. Aspergillus niger and Paecilomyces javanicus were grown on modified Czapek-Dox medium amended with glycerol 2-phosphate (G2P) as sole P source and uranium nitrate. Both organisms showed reduced growth on uranium-containing media but were able to extensively precip...

The following studies were initially undertaken to investigate the quantitative relations of potassium changes in blood and muscle after adrenalectomy in the light of the work done on the nature of potassium accumulation (Boyle & Conway, 1941). In the course of the investigation the mean values of the known and quantitatively important substances in muscle and plasma were determined. These incl...